biostudies-literatureUnknown9(4)Pons SSociété de Réanimation de Langue FrançaiseEuropean Society of Intensive Care Medicine<h4>Objectives</h4>Sepsis is defined as the host's inflammatory response to a life-threatening infection. The endothelium is implicated in immunoregulation during sepsis. Macrolides have been proposed to display immunomodulatory properties. The goal of this study was to analyze whether macrolides can exert immunomodulation of endothelial cells (ECs) in an experimental model of sepsis.<h4>Methods</h4>Human ECs were stimulated by proinflammatory cytokines and lipopolysaccharide before exposure to macrolides. ECs phenotypes were analyzed by flow cytometry. Cocultures of ECs and peripheral blood mononuclear cells (PBMCs) were performed to study the ECs ability to alter T-cell viability and differentiation in the presence of macrolides. Soluble factor production was assessed.<h4>Results</h4>ECsImmunity, inflammation and disease1656-1669https://www.ebi.ac.uk/biostudies/studies/S-EPMC8589380biostudies-literatureImmunomodulation of endothelial cells induced by macrolide therapy in a model of septic stimulation.PMC8589380Arrii EPons SArnaud MLoiselle MMooney NLion JZafrani LCohen SFerry JNouacer MfalseImmunomodulation of endothelial cells induced by macrolide therapy in a model of septic stimulation.<h4>Objectives</h4>Sepsis is defined as the host's inflammatory response to a life-threatening infection. The endothelium is implicated in immunoregulation during sepsis. Macrolides have been proposed to display immunomodulatory properties. The goal of this study was to analyze whether macrolides can exert immunomodulation of endothelial cells (ECs) in an experimental model of sepsis.<h4>Methods</h4>Human ECs were stimulated by proinflammatory cytokines and lipopolysaccharide before exposure to macrolides. ECs phenotypes were analyzed by flow cytometry. Cocultures of ECs and peripheral blood mononuclear cells (PBMCs) were performed to study the ECs ability to alter T-cell viability and differentiation in the presence of macrolides. Soluble factor production was assessed.<h4>Results</h4>ECs2021-01-01T00:00:00Z2021 Dec2025-04-19T05:05:11.233Z2022-02-11T13:01:48.241ZS-EPMC85893803463617910.1002/iid3.518