{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Korpanty J"],"funding":["U.S. Department of Energy","United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office","NIH HHS","National Science Foundation"],"pagination":["6568"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8589985"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Herein, phase transitions of a class of thermally-responsive polymers, namely a homopolymer, diblock, and triblock copolymer, were studied to gain mechanistic insight into nanoscale assembly dynamics via variable temperature liquid-cell transmission electron microscopy (VT-LCTEM) correlated with variable temperature small angle X-ray scattering (VT-SAXS). We study thermoresponsive poly(diethylene glycol methyl ether methacrylate) (PDEGMA)-based block copolymers and mitigate sample damage by screening electron flux and solvent conditions during LCTEM and by evaluating polymer survival via post-mortem matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). Our multimodal approach, utilizing VT-LCTEM with MS validation and VT-SAXS, is generalizable across polymeric systems and can be used to directly image solvated nanoscale structures and thermally-induced transitions. Our strategy of correlating VT-SAXS with VT-LCTEM provided direct insight into transient nanoscale intermediates formed during the thermally-triggered morphological transformation of a PDEGMA-based triblock. Notably, we observed the temperature-triggered formation and slow relaxation of core-shell particles with complex microphase separation in the core by both VT-SAXS and VT-LCTEM."],"journal":["Nature communications"],"pubmed_title":["Thermoresponsive polymer assemblies via variable temperature liquid-phase transmission electron microscopy and small angle X-ray scattering."],"pmcid":["PMC8589985"],"funding_grant_id":["W911NF-17-1-0326","DE-AC02-06CH11357","S10 OD026871","NSF DMR1720139","CHE-MSN 1905270","NSF ECCS-1542205"],"pubmed_authors":["Gianneschi NC","Korpanty J","Weigand S","Parent LR","Hampu N"],"additional_accession":[]},"is_claimable":false,"name":"Thermoresponsive polymer assemblies via variable temperature liquid-phase transmission electron microscopy and small angle X-ray scattering.","description":"Herein, phase transitions of a class of thermally-responsive polymers, namely a homopolymer, diblock, and triblock copolymer, were studied to gain mechanistic insight into nanoscale assembly dynamics via variable temperature liquid-cell transmission electron microscopy (VT-LCTEM) correlated with variable temperature small angle X-ray scattering (VT-SAXS). We study thermoresponsive poly(diethylene glycol methyl ether methacrylate) (PDEGMA)-based block copolymers and mitigate sample damage by screening electron flux and solvent conditions during LCTEM and by evaluating polymer survival via post-mortem matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). Our multimodal approach, utilizing VT-LCTEM with MS validation and VT-SAXS, is generalizable across polymeric systems and can be used to directly image solvated nanoscale structures and thermally-induced transitions. Our strategy of correlating VT-SAXS with VT-LCTEM provided direct insight into transient nanoscale intermediates formed during the thermally-triggered morphological transformation of a PDEGMA-based triblock. Notably, we observed the temperature-triggered formation and slow relaxation of core-shell particles with complex microphase separation in the core by both VT-SAXS and VT-LCTEM.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2024-12-04T12:24:57.092Z","creation":"2022-02-11T12:56:28.87Z"},"accession":"S-EPMC8589985","cross_references":{"pubmed":["34772926"],"doi":["10.1038/s41467-021-26773-z"]}}