biostudies-literatureLu Jthe Key Research Program of Frontier Sciences of the Chinese Academy of SciencesNational Natural Science Foundation of Chinathe Yantai Region and College Integration Development Projectthe National Natural Science Foundation of China966-970https://www.ebi.ac.uk/biostudies/studies/S-EPMC8593523biostudies-literatureUnknown22(11)Hydrogen (H₂)-rich water, an apparent source of molecular H₂, is an emerging functional drink with many purported benefits for human health (Yang et al., 2020; Ostojic, 2021). The preventive and therapeutic effects of H₂ on various pathological processes have been intensively investigated in numerous clinical trials; it is commonly believed that the beneficial effects are mainly attributed to its selective antioxidant and anti-inflammatory properties (Lee et al., 2015; Ohta, 2015; LeBaron et al., 2019; Qiu et al., 2020). In recent years, a handful of rodent studies revealed that exogenous H₂ can affect the gut microbiota (Sha et al., 2018; Valdes et al., 2018). For example, H₂ was reported to induce a higher abundance of butyrate-produJournal of Zhejiang University. Science. BResponse of <i>Escherichia coli</i> to hydrogen nanobubbles: an in vitro evaluation using synchrotron infrared spectroscopy.PMC8593523U1732130QYZDJSSW-SLH0192021XDRHXMPT28Zheng JLi XHu JLu JLi BWang YCheng JfalseResponse of <i>Escherichia coli</i> to hydrogen nanobubbles: an in vitro evaluation using synchrotron infrared spectroscopy.Hydrogen (H₂)-rich water, an apparent source of molecular H₂, is an emerging functional drink with many purported benefits for human health (Yang et al., 2020; Ostojic, 2021). The preventive and therapeutic effects of H₂ on various pathological processes have been intensively investigated in numerous clinical trials; it is commonly believed that the beneficial effects are mainly attributed to its selective antioxidant and anti-inflammatory properties (Lee et al., 2015; Ohta, 2015; LeBaron et al., 2019; Qiu et al., 2020). In recent years, a handful of rodent studies revealed that exogenous H₂ can affect the gut microbiota (Sha et al., 2018; Valdes et al., 2018). For example, H₂ was reported to induce a higher abundance of butyrate-produ2021-01-01T00:00:00Z2021 Nov2025-04-04T21:33:43.941Z2022-02-11T13:27:13.902ZS-EPMC85935233478322710.1631/jzus.B210040710.1631/jzus.b2100407