biostudies-literatureSharma NCouncil of Scientific and Industrial ResearchDepartment of Science and Technology, Ministry of Science and Technology30241-30259https://www.ebi.ac.uk/biostudies/studies/S-EPMC8600527biostudies-literatureUnknown6(45)<i>Colebrookea oppositifolia</i> is a folkloric medicinal plant, well known for its tremendous medicinal properties such as curing epilepsy, ulcers, and urinary problems. The aim of the present study was to apply the dereplication strategy on the ethanol extract of <i>C. oppositifolia</i> with potent anti-inflammatory activity for the rapid identification and isolation of novel bioactive molecules to aid the drug discovery process. An integrated approach using liquid chromatography-mass spectrometry (LCMS) followed by preparative high-performance liquid chromatography (HPLC) was used for the isolation of potent molecules from the anti-inflammatory extract of <i>C. oppositifolia</i> <i>.</i> Purity of the compounds (>98.5%) was established by HPLC, and identification was carried out by NMR ACS omegaDereplication Based Strategy for Rapid Identification and Isolation of a Novel Anti-inflammatory Flavonoid by LCMS/MS from <i>Colebrookea oppositifolia</i>.PMC8600527BSC-0110Sharma NSatti NKSharma AGupta SAhmed ZKhajuria VVerma MKLone NAPaul SMeena SRKumar CfalseDereplication Based Strategy for Rapid Identification and Isolation of a Novel Anti-inflammatory Flavonoid by LCMS/MS from <i>Colebrookea oppositifolia</i>.<i>Colebrookea oppositifolia</i> is a folkloric medicinal plant, well known for its tremendous medicinal properties such as curing epilepsy, ulcers, and urinary problems. The aim of the present study was to apply the dereplication strategy on the ethanol extract of <i>C. oppositifolia</i> with potent anti-inflammatory activity for the rapid identification and isolation of novel bioactive molecules to aid the drug discovery process. An integrated approach using liquid chromatography-mass spectrometry (LCMS) followed by preparative high-performance liquid chromatography (HPLC) was used for the isolation of potent molecules from the anti-inflammatory extract of <i>C. oppositifolia</i> <i>.</i> Purity of the compounds (>98.5%) was established by HPLC, and identification was carried out by NMR 2021-01-01T00:00:00Z2021 Nov2025-04-18T12:09:05.829Z2022-02-11T13:14:02.93ZS-EPMC86005273480565810.1021/acsomega.1c01837