{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chaudhary S"],"funding":["NINDS NIH HHS","National Institutes of Health"],"pagination":["108787"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8608713"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["212"],"pubmed_abstract":["Recently, we reported β-cleavage of the prion protein (PrP<sup>C</sup>) in human ocular tissues. Here, we explored whether this is unique to the human eye, and its functional implications. A comparison of the cleavage pattern of PrP<sup>C</sup> in human ocular tissues with common nocturnal and diurnal animals revealed mainly β-cleavage in humans, and mostly full-length PrP<sup>C</sup> in animal retinas. Soluble FL PrP<sup>C</sup> and N-terminal fragment (N2) released from β-cleavage was observed in the aqueous and vitreous humor (AH & VH). Expression of human PrP<sup>C</sup> in ARPE-19 cells, a human retinal pigmented epithelial cell line, also showed β-cleaved PrP<sup>C</sup>. Surprisingly, β-cleavage was not altered by a variety of insults, including oxidative stress, suggesting a unique"],"journal":["Experimental eye research"],"pubmed_title":["β-Cleavage of the prion protein in the human eye: Implications for the spread of infectious prions and human ocular disorders."],"pmcid":["PMC8608713"],"funding_grant_id":["R01 NS092145"],"pubmed_authors":["Lindner E","Wise AS","Chaudhary S","Singh N","Kritikos AE","Ashok A","Rana NA"],"additional_accession":[]},"is_claimable":false,"name":"β-Cleavage of the prion protein in the human eye: Implications for the spread of infectious prions and human ocular disorders.","description":"Recently, we reported β-cleavage of the prion protein (PrP<sup>C</sup>) in human ocular tissues. Here, we explored whether this is unique to the human eye, and its functional implications. A comparison of the cleavage pattern of PrP<sup>C</sup> in human ocular tissues with common nocturnal and diurnal animals revealed mainly β-cleavage in humans, and mostly full-length PrP<sup>C</sup> in animal retinas. Soluble FL PrP<sup>C</sup> and N-terminal fragment (N2) released from β-cleavage was observed in the aqueous and vitreous humor (AH & VH). Expression of human PrP<sup>C</sup> in ARPE-19 cells, a human retinal pigmented epithelial cell line, also showed β-cleaved PrP<sup>C</sup>. Surprisingly, β-cleavage was not altered by a variety of insults, including oxidative stress, suggesting a unique","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-05T11:27:28.061Z","creation":"2025-04-05T11:27:28.061Z"},"accession":"S-EPMC8608713","cross_references":{"pubmed":["34624335"],"doi":["10.1016/j.exer.2021.108787"]}}