biostudies-literatureMassey VNIDDK NIH HHSMedical Research CouncilNIAAA NIH HHS1725-1740.e2https://www.ebi.ac.uk/biostudies/studies/S-EPMC8613537biostudies-literatureUnknown160(5)<h4>Background & aims</h4>We recently showed that alcoholic hepatitis (AH) is characterized by dedifferentiation of hepatocytes and loss of mature functions. Glucose metabolism is tightly regulated in healthy hepatocytes. We hypothesize that AH may lead to metabolic reprogramming of the liver, including dysregulation of glucose metabolism.<h4>Methods</h4>We performed integrated metabolomic and transcriptomic analyses of liver tissue from patients with AH or alcoholic cirrhosis or normal liver tissue from hepatic resection. Focused analyses of chromatin immunoprecipitation coupled to DNA sequencing was performed. Functional in vitro studies were performed in primary rat and human hepatocytes and HepG2 cells.<h4>Results</h4>Patients with AH exhibited specific changes in the levels of intermeGastroenterologyIntegrated Multiomics Reveals Glucose Use Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis.PMC8613537P30 DK120531R24 AA025017R01 AA018873F31 AA024969U01 AA026972MR/R023026/1U01 AA021908R01 DK110355U01 AA026264P50 AA027054U01 AA026978T32 AA007463Morales-Arraez DAltamirano JMassey VCameron AArgemi JCaballeria JVadigepalli RDubuquoy LLouvet AOdena GShah VVillanueva CMillan CMello ABataller RGarcia-Rocha MSancho-Bru PParrish ASun ZMathurin PAdrover AMorgan TRMann JAlvarado-Tapias EMorales-Ibanez OAffo SCao SMoreno MMartinez CSnider NGuinovart JfalseIntegrated Multiomics Reveals Glucose Use Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis.<h4>Background & aims</h4>We recently showed that alcoholic hepatitis (AH) is characterized by dedifferentiation of hepatocytes and loss of mature functions. Glucose metabolism is tightly regulated in healthy hepatocytes. We hypothesize that AH may lead to metabolic reprogramming of the liver, including dysregulation of glucose metabolism.<h4>Methods</h4>We performed integrated metabolomic and transcriptomic analyses of liver tissue from patients with AH or alcoholic cirrhosis or normal liver tissue from hepatic resection. Focused analyses of chromatin immunoprecipitation coupled to DNA sequencing was performed. Functional in vitro studies were performed in primary rat and human hepatocytes and HepG2 cells.<h4>Results</h4>Patients with AH exhibited specific changes in the levels of interme2021-01-01T00:00:00Z2021 Apr2026-05-09T04:12:58.096Z2025-02-18T23:36:04.246ZS-EPMC86135373330977810.1053/j.gastro.2020.12.008