{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["38(2)"],"submitter":["Scotto d'Abusco AS"],"pubmed_abstract":["Enterotoxigenic Bacteroides fragilis (ETBF) strains are associated with diarrheal disease in children. These strains produce a zinc metalloprotease enterotoxin, or fragilysin, that can be detected by a cytotoxicity assay with HT-29 cells. Recently, three different isoforms or variants of the enterotoxin gene, designated bft-1, bft-2, and bft-3, have been identified and sequenced. We used restriction fragment length polymorphism analysis of the PCR-amplified enterotoxin gene to detect the isoforms bft-1 and bft-2 or bft-3 borne by ETBF. By sequencing the portion of the bft gene corresponding to the mature toxin in some strains and applying allele-specific PCR for strains categorized as bft-2 or bft-3, we found in our collection two strains harboring bft-3, a variant that had been described "],"journal":["Journal of clinical microbiology"],"pagination":["607-12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC86157"],"repository":["biostudies-literature"],"pubmed_title":["The alleles of the bft gene are distributed differently among enterotoxigenic Bacteroides fragilis strains from human sources and can be present in double copies."],"pmcid":["PMC86157"],"pubmed_authors":["Covacci A","Censini S","Scotto d'Abusco AS","Pantosti A","Del Grosso M"],"additional_accession":[]},"is_claimable":false,"name":"The alleles of the bft gene are distributed differently among enterotoxigenic Bacteroides fragilis strains from human sources and can be present in double copies.","description":"Enterotoxigenic Bacteroides fragilis (ETBF) strains are associated with diarrheal disease in children. These strains produce a zinc metalloprotease enterotoxin, or fragilysin, that can be detected by a cytotoxicity assay with HT-29 cells. Recently, three different isoforms or variants of the enterotoxin gene, designated bft-1, bft-2, and bft-3, have been identified and sequenced. We used restriction fragment length polymorphism analysis of the PCR-amplified enterotoxin gene to detect the isoforms bft-1 and bft-2 or bft-3 borne by ETBF. By sequencing the portion of the bft gene corresponding to the mature toxin in some strains and applying allele-specific PCR for strains categorized as bft-2 or bft-3, we found in our collection two strains harboring bft-3, a variant that had been described ","dates":{"release":"2000-01-01T00:00:00Z","publication":"2000 Feb","modification":"2025-04-05T10:13:58.748Z","creation":"2019-03-27T00:18:21Z"},"accession":"S-EPMC86157","cross_references":{"pubmed":["10655354"],"doi":["10.1128/JCM.38.2.607-612.2000"]}}