{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chauvier A"],"funding":["HHS | National Institutes of Health","NIGMS NIH HHS"],"pagination":["e2109026118"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8617461"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["118(47)"],"pubmed_abstract":["Cotranscriptional RNA folding is widely assumed to influence the timely control of gene expression, but our understanding remains limited. In bacteria, the fluoride (F<sup>-</sup>)-sensing riboswitch is a transcriptional control element essential to defend against toxic F<sup>-</sup> levels. Using this model riboswitch, we find that its ligand F<sup>-</sup> and essential bacterial transcription factor NusA compete to bind the cotranscriptionally folding RNA, opposing each other's modulation of downstream pausing and termination by RNA polymerase. Single-molecule fluorescence assays probing active transcription elongation complexes discover that NusA unexpectedly binds highly reversibly, frequently interrogating the complex for emerging, cotranscriptionally folding RNA duplexes. NusA thus f"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation."],"pmcid":["PMC8617461"],"funding_grant_id":["R01 GM118524","R35 GM131922","GM062357","GM131922","GM118524","R01 GM062357"],"pubmed_authors":["Walter NG","Ajmera P","Chauvier A","Yadav R"],"additional_accession":[]},"is_claimable":false,"name":"Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation.","description":"Cotranscriptional RNA folding is widely assumed to influence the timely control of gene expression, but our understanding remains limited. In bacteria, the fluoride (F<sup>-</sup>)-sensing riboswitch is a transcriptional control element essential to defend against toxic F<sup>-</sup> levels. Using this model riboswitch, we find that its ligand F<sup>-</sup> and essential bacterial transcription factor NusA compete to bind the cotranscriptionally folding RNA, opposing each other's modulation of downstream pausing and termination by RNA polymerase. Single-molecule fluorescence assays probing active transcription elongation complexes discover that NusA unexpectedly binds highly reversibly, frequently interrogating the complex for emerging, cotranscriptionally folding RNA duplexes. NusA thus f","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-04T07:51:15.483Z","creation":"2025-04-04T07:51:15.483Z"},"accession":"S-EPMC8617461","cross_references":{"pubmed":["34782462"],"doi":["10.1073/pnas.2109026118"]}}