{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":45,"searchCount":0},"additional":{"submitter":["Chakkarapani SK"],"funding":["ministry of science and ict (kr)","ministry of science and ict","ministry of education"],"pagination":["398"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8628397"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["<h4>Background</h4>Nanoparticles have been used for biomedical applications, including drug delivery, diagnosis, and imaging based on their unique properties derived from small size and large surface-to-volume ratio. However, concerns regarding unexpected toxicity due to the localization of nanoparticles in the cells are growing. Herein, we quantified the number of cell-internalized nanoparticles and monitored their cellular localization, which are critical factors for biomedical applications of nanoparticles.<h4>Methods</h4>This study investigates the intracellular trafficking of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO<sub>2</sub>(RITC)] in various live single cells, such as HEK293, NIH3T3, and RAW 264.7 cells, using site-specific direct stochastic optical reconstruction microscopy (dSTORM). The time-dependent subdiffraction-limit spatial resolution of the dSTORM method allowed intracellular site-specific quantification and tracking of MNPs@SiO<sub>2</sub>(RITC).<h4>Results</h4>The MNPs@SiO<sub>2</sub>(RITC) were observed to be highly internalized in RAW 264.7 cells, compared to the HEK293 and NIH3T3 cells undergoing single-particle analysis. In addition, MNPs@SiO<sub>2</sub>(RITC) were internalized within the nuclei of RAW 264.7 and HEK293 cells but were not detected in the nuclei of NIH3T3 cells. Moreover, because of the treatment of the MNPs@SiO<sub>2</sub>(RITC), more micronuclei were detected in RAW 264.7 cells than in other cells.<h4>Conclusion</h4>The sensitive and quantitative evaluations of MNPs@SiO<sub>2</sub>(RITC) at specific sites in three different cells using a combination of dSTORM, transcriptomics, and molecular biology were performed. These findings highlight the quantitative differences in the uptake efficiency of MNPs@SiO<sub>2</sub>(RITC) and ultra-sensitivity, varying according to the cell types as ascertained by subdiffraction-limit super-resolution microscopy."],"journal":["Journal of nanobiotechnology"],"pubmed_title":["Quantifying intracellular trafficking of silica-coated magnetic nanoparticles in live single cells by site-specific direct stochastic optical reconstruction microscopy."],"pmcid":["PMC8628397"],"funding_grant_id":["2019R1A2C12002556","2020R1A4A4079722"],"pubmed_authors":["Kang SH","Lee S","Chakkarapani SK","Shin TH","Lee G","Park KS"],"view_count":["45"],"additional_accession":[]},"is_claimable":false,"name":"Quantifying intracellular trafficking of silica-coated magnetic nanoparticles in live single cells by site-specific direct stochastic optical reconstruction microscopy.","description":"<h4>Background</h4>Nanoparticles have been used for biomedical applications, including drug delivery, diagnosis, and imaging based on their unique properties derived from small size and large surface-to-volume ratio. However, concerns regarding unexpected toxicity due to the localization of nanoparticles in the cells are growing. Herein, we quantified the number of cell-internalized nanoparticles and monitored their cellular localization, which are critical factors for biomedical applications of nanoparticles.<h4>Methods</h4>This study investigates the intracellular trafficking of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO<sub>2</sub>(RITC)] in various live single cells, such as HEK293, NIH3T3, and RAW 264.7 cells, using site-specific direct stochastic optical reconstruction microscopy (dSTORM). The time-dependent subdiffraction-limit spatial resolution of the dSTORM method allowed intracellular site-specific quantification and tracking of MNPs@SiO<sub>2</sub>(RITC).<h4>Results</h4>The MNPs@SiO<sub>2</sub>(RITC) were observed to be highly internalized in RAW 264.7 cells, compared to the HEK293 and NIH3T3 cells undergoing single-particle analysis. In addition, MNPs@SiO<sub>2</sub>(RITC) were internalized within the nuclei of RAW 264.7 and HEK293 cells but were not detected in the nuclei of NIH3T3 cells. Moreover, because of the treatment of the MNPs@SiO<sub>2</sub>(RITC), more micronuclei were detected in RAW 264.7 cells than in other cells.<h4>Conclusion</h4>The sensitive and quantitative evaluations of MNPs@SiO<sub>2</sub>(RITC) at specific sites in three different cells using a combination of dSTORM, transcriptomics, and molecular biology were performed. These findings highlight the quantitative differences in the uptake efficiency of MNPs@SiO<sub>2</sub>(RITC) and ultra-sensitivity, varying according to the cell types as ascertained by subdiffraction-limit super-resolution microscopy.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2024-12-03T14:53:22.6Z","creation":"2022-02-11T13:36:23.071Z"},"accession":"S-EPMC8628397","cross_references":{"pubmed":["34844629"],"doi":["10.1186/s12951-021-01147-1"]}}