{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Faivre B"],"funding":["Agence Nationale de la Recherche","Foundation for the National Institutes of Health","NIGMS NIH HHS"],"pagination":["2278-2289"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8632129"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(12)"],"pubmed_abstract":["Dihydrouridine (D) is a tRNA-modified base conserved throughout all kingdoms of life and assuming an important structural role. The conserved dihydrouridine synthases (Dus) carries out D-synthesis. DusA, DusB and DusC are bacterial members, and their substrate specificity has been determined in <i>Escherichia coli</i>. DusA synthesizes D20/D20a while DusB and DusC are responsible for the synthesis of D17 and D16, respectively. Here, we characterize the function of the unique <i>dus</i> gene encoding a DusB detected in Mollicutes, which are bacteria that evolved from a common Firmicute ancestor via massive genome reduction. Using <i>in vitro</i> activity tests as well as <i>in vivo E. coli</i> complementation assays with the enzyme from <i>Mycoplasma capricolum</i> (DusB<i><sub>MCap</sub></"],"journal":["RNA biology"],"pubmed_title":["Dihydrouridine synthesis in tRNAs is under reductive evolution in Mollicutes."],"pmcid":["PMC8632129"],"funding_grant_id":["R01 GM070641","ANR-15-CE11-0004-01","ANR-11-LABX-0011","GM70641"],"pubmed_authors":["De Crecy-Lagard V","Bregeon D","Faivre B","Fontecave M","Vo CD","Fakroun S","Pecqueur L","Lombard M","Guerineau V","Hamdane D","Goyenvalle C"],"additional_accession":[]},"is_claimable":false,"name":"Dihydrouridine synthesis in tRNAs is under reductive evolution in Mollicutes.","description":"Dihydrouridine (D) is a tRNA-modified base conserved throughout all kingdoms of life and assuming an important structural role. The conserved dihydrouridine synthases (Dus) carries out D-synthesis. DusA, DusB and DusC are bacterial members, and their substrate specificity has been determined in <i>Escherichia coli</i>. DusA synthesizes D20/D20a while DusB and DusC are responsible for the synthesis of D17 and D16, respectively. Here, we characterize the function of the unique <i>dus</i> gene encoding a DusB detected in Mollicutes, which are bacteria that evolved from a common Firmicute ancestor via massive genome reduction. Using <i>in vitro</i> activity tests as well as <i>in vivo E. coli</i> complementation assays with the enzyme from <i>Mycoplasma capricolum</i> (DusB<i><sub>MCap</sub></","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2026-04-08T17:50:26.825Z","creation":"2025-04-05T15:22:41.811Z"},"accession":"S-EPMC8632129","cross_references":{"pubmed":["33685366"],"doi":["10.1080/15476286.2021.1899653"]}}