{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(11)"],"submitter":["Kacal M"],"funding":["Vetenskapsrådet","Cancerfonden","Ragnar Söderbergs stiftelse"],"pubmed_abstract":["Autophagic pathways are regulated mechanisms that play important roles in lysosome-mediated cellular degradation. Yet, the contribution of different autophagic pathways in lysosomal targeting, and characterization of the extent and specificity in their degradome remains largely uncharacterized. By undertaking a multiplex quantitative mass spectrometry approach, we have previously analyzed the lysosomal proteome during chaperone-mediated autophagy (CMA)-stimulated conditions in cancer cells. Here, we have extended our multiplex quantitative mass spectrometry and bioinformatics analysis on the proteome from isolated lysosomes to gain a comprehensive view of the temporal enriched lysosomal content upon non-macroautophagy-activated conditions. In parallel, we describe the functional dependency"],"journal":["Autophagy"],"pagination":["3865-3874"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8632328"],"repository":["biostudies-literature"],"pubmed_title":["Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting."],"pmcid":["PMC8632328"],"pubmed_authors":["Hao Y","Norberg E","Vakifahmetoglu-Norberg H","Zhang B","Kacal M"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative proteomic analysis of temporal lysosomal proteome and the impact of the KFERQ-like motif and LAMP2A in lysosomal targeting.","description":"Autophagic pathways are regulated mechanisms that play important roles in lysosome-mediated cellular degradation. Yet, the contribution of different autophagic pathways in lysosomal targeting, and characterization of the extent and specificity in their degradome remains largely uncharacterized. By undertaking a multiplex quantitative mass spectrometry approach, we have previously analyzed the lysosomal proteome during chaperone-mediated autophagy (CMA)-stimulated conditions in cancer cells. Here, we have extended our multiplex quantitative mass spectrometry and bioinformatics analysis on the proteome from isolated lysosomes to gain a comprehensive view of the temporal enriched lysosomal content upon non-macroautophagy-activated conditions. In parallel, we describe the functional dependency","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2026-05-09T11:40:46.683Z","creation":"2022-02-11T14:19:39.222Z"},"accession":"S-EPMC8632328","cross_references":{"pubmed":["33446043"],"doi":["10.1080/15548627.2021.1876343"]}}