biostudies-literatureIocco-Corena PCommonwealth Scientific and Industrial Research OrganisationWine Australia6995https://www.ebi.ac.uk/biostudies/studies/S-EPMC8632994biostudies-literatureUnknown12(1)Plant genetic sex determinants that mediate the transition to dioecy are predicted to be diverse, as this type of mating system independently evolved multiple times in angiosperms. Wild Vitis species are dioecious with individuals producing morphologically distinct female or male flowers; whereas, modern domesticated Vitis vinifera cultivars form hermaphrodite flowers capable of self-pollination. Here, we identify the VviPLATZ1 transcription factor as a key candidate female flower morphology factor that localizes to the Vitis SEX-DETERMINING REGION. The expression pattern of this gene correlates with the formation reflex stamens, a prominent morphological phenotype of female flowers. After generating CRISPR/Cas9 gene-edited alleles in a hermaphrodite genotype, phenotype analysis shows thatNature communicationsVviPLATZ1 is a major factor that controls female flower morphology determination in grapevine.PMC8632994CSP01/06CSP 06/01Torregrosa LMackenzie DIocco-Corena PSmith HMChaib JThomas MRfalseVviPLATZ1 is a major factor that controls female flower morphology determination in grapevine.Plant genetic sex determinants that mediate the transition to dioecy are predicted to be diverse, as this type of mating system independently evolved multiple times in angiosperms. Wild Vitis species are dioecious with individuals producing morphologically distinct female or male flowers; whereas, modern domesticated Vitis vinifera cultivars form hermaphrodite flowers capable of self-pollination. Here, we identify the VviPLATZ1 transcription factor as a key candidate female flower morphology factor that localizes to the Vitis SEX-DETERMINING REGION. The expression pattern of this gene correlates with the formation reflex stamens, a prominent morphological phenotype of female flowers. After generating CRISPR/Cas9 gene-edited alleles in a hermaphrodite genotype, phenotype analysis shows that2021-01-01T00:00:00Z2021 Nov2026-05-09T17:08:48.042Z2025-05-29T20:11:23.608ZS-EPMC86329943484871410.1038/s41467-021-27259-8