biostudies-literatureVelez IDWellcome Trust1327https://www.ebi.ac.uk/biostudies/studies/S-EPMC8637243biostudies-literatureUnknown8Background: Dengue, chikungunya and Zika are viral infections transmitted by Aedes aegypti mosquitoes, and present major public health challenges in tropical regions. Traditional vector control methods have been ineffective at halting disease transmission. The World Mosquito Program has developed a novel approach to arbovirus control using Ae. aegypti stably transfected with the Wolbachia bacterium, which have significantly reduced ability to transmit dengue, Zika and chikungunya in laboratory experiments. Field releases in eight countries have demonstrated Wolbachia establishment in local Ae. aegypti populations. Methods: We describe a pragmatic approach to measuring the epidemiological impact of city-wide Wolbachia deployments in Bello and Medellín, Colombia. First, an interrupted time-sF1000ResearchThe impact of city-wide deployment of Wolbachia-carrying mosquitoes on arboviral disease incidence in Medellin and Bello, Colombia: study protocol for an interrupted time-series analysis and a test-negative design study.PMC8637243102591/Z/13/AKutcher SCVelez IDZuluaga LO'Neill SLMejia MCTanamas SKGonzalez SPulido HPatino ACDuque SLMartinez LSantacruz EAnders KLArbelaez MPJewell NPSimmons CPBarajas JUribe AfalseThe impact of city-wide deployment of Wolbachia-carrying mosquitoes on arboviral disease incidence in Medellin and Bello, Colombia: study protocol for an interrupted time-series analysis and a test-negative design study.Background: Dengue, chikungunya and Zika are viral infections transmitted by Aedes aegypti mosquitoes, and present major public health challenges in tropical regions. Traditional vector control methods have been ineffective at halting disease transmission. The World Mosquito Program has developed a novel approach to arbovirus control using Ae. aegypti stably transfected with the Wolbachia bacterium, which have significantly reduced ability to transmit dengue, Zika and chikungunya in laboratory experiments. Field releases in eight countries have demonstrated Wolbachia establishment in local Ae. aegypti populations. Methods: We describe a pragmatic approach to measuring the epidemiological impact of city-wide Wolbachia deployments in Bello and Medellín, Colombia. First, an interrupted time-s2019-01-01T00:00:00Z20192025-04-22T08:09:35.561Z2022-02-11T14:02:41.491ZS-EPMC86372433490023710.12688/f1000research.19858.1