biostudies-literatureDevall MAMNIDDK NIH HHSNIH NIDDKNCI NIH HHSNIH NCI1089-1100https://www.ebi.ac.uk/biostudies/studies/S-EPMC8639779biostudies-literatureUnknown14(12)Mechanisms underlying aspirin chemoprevention of colorectal cancer remain unclear. Prior studies have been limited because of the inability of preclinical models to recapitulate human normal colon epithelium or cellular heterogeneity present in mucosal biopsies. To overcome some of these obstacles, we performed <i>in vitro</i> aspirin treatment of colon organoids derived from normal mucosal biopsies to reveal transcriptional networks relevant to aspirin chemoprevention. Colon organoids derived from 38 healthy individuals undergoing endoscopy were treated with 50 μmol/L aspirin or vehicle control for 72 hours and subjected to bulk RNA sequencing. Paired regression analysis using DESeq2 identified differentially expressed genes (DEG) associated with aspirin treatment. Cellular composition waCancer prevention research (Philadelphia, Pa.)Transcriptome-wide <i>In Vitro</i> Effects of Aspirin on Patient-derived Normal Colon Organoids.PMC8639779K01 DK120742R01 CA211184R01 CA257523R01 CA201407R01 CA137178R35 CA253185L30 CA209764CA254314R01 CA143237F32 CA254314R01 CA204279R01 CA245314Eaton SAli MWChan ATTakacsi-Nagy OMo JPowell SMDrew DADevall MAMBryant JJennelle LTDampier CHDiez-Obrero VMoreno VPeters UPlummer SJKedrin DYilmaz OHCasey GZerjav DCfalseTranscriptome-wide <i>In Vitro</i> Effects of Aspirin on Patient-derived Normal Colon Organoids.Mechanisms underlying aspirin chemoprevention of colorectal cancer remain unclear. Prior studies have been limited because of the inability of preclinical models to recapitulate human normal colon epithelium or cellular heterogeneity present in mucosal biopsies. To overcome some of these obstacles, we performed <i>in vitro</i> aspirin treatment of colon organoids derived from normal mucosal biopsies to reveal transcriptional networks relevant to aspirin chemoprevention. Colon organoids derived from 38 healthy individuals undergoing endoscopy were treated with 50 μmol/L aspirin or vehicle control for 72 hours and subjected to bulk RNA sequencing. Paired regression analysis using DESeq2 identified differentially expressed genes (DEG) associated with aspirin treatment. Cellular composition wa2021-01-01T00:00:00Z2021 Dec2026-05-09T20:13:38.679Z2025-02-19T00:56:31.267ZS-EPMC86397793438962910.1158/1940-6207.capr-21-004110.1158/1940-6207.CAPR-21-0041