{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(21)"],"submitter":["van Hoof A"],"pubmed_abstract":["One of two general pathways of mRNA decay in the yeast Saccharomyces cerevisiae occurs by deadenylation followed by 3'-to-5' degradation of the mRNA body. Previous results have shown that this degradation requires components of the exosome and the Ski2p, Ski3p, and Ski8p proteins, which were originally identified due to their superkiller phenotype. In this work, we demonstrate that deletion of the SKI7 gene, which encodes a putative GTPase, also causes a defect in 3'-to-5' degradation of mRNA. Deletion of SKI7, like deletion of SKI2, SKI3, or SKI8, does not affect various RNA-processing reactions of the exosome. In addition, we show that a mutation in the SKI4 gene also causes a defect in 3'-to-5' mRNA degradation. We show that the SKI4 gene is identical to the CSL4 gene, which encodes a c"],"journal":["Molecular and cellular biology"],"pagination":["8230-43"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC86432"],"repository":["biostudies-literature"],"pubmed_title":["Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA."],"pmcid":["PMC86432"],"pubmed_authors":["van Hoof A","Staples RR","Baker RE","Parker R"],"additional_accession":[]},"is_claimable":false,"name":"Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA.","description":"One of two general pathways of mRNA decay in the yeast Saccharomyces cerevisiae occurs by deadenylation followed by 3'-to-5' degradation of the mRNA body. Previous results have shown that this degradation requires components of the exosome and the Ski2p, Ski3p, and Ski8p proteins, which were originally identified due to their superkiller phenotype. In this work, we demonstrate that deletion of the SKI7 gene, which encodes a putative GTPase, also causes a defect in 3'-to-5' degradation of mRNA. Deletion of SKI7, like deletion of SKI2, SKI3, or SKI8, does not affect various RNA-processing reactions of the exosome. In addition, we show that a mutation in the SKI4 gene also causes a defect in 3'-to-5' mRNA degradation. We show that the SKI4 gene is identical to the CSL4 gene, which encodes a c","dates":{"release":"2000-01-01T00:00:00Z","publication":"2000 Nov","modification":"2025-04-05T10:14:18.688Z","creation":"2019-03-27T00:18:21Z"},"accession":"S-EPMC86432","cross_references":{"pubmed":["11027292"],"doi":["10.1128/MCB.20.21.8230-8243.2000"]}}