{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(6)"],"submitter":["Hall VG"],"funding":["the Ajmera Transplant Centre"],"pubmed_abstract":["The optimal management of immunosuppression in transplant patients infected with COVID-19 is unknown. We performed an in vitro study to determine the effect of individual immunosuppressive agents on SARS-CoV-2-specific T-cell cytokine expression. Convalescent peripheral blood mononuclear cells from eleven non-immunosuppressed patients with COVID-19 were preincubated with clinically relevant concentrations of immunosuppressive drugs (tacrolimus, mycophenolate, sirolimus, prednisone) and then stimulated with a SARS-CoV-2 peptide pool. Supernatants were analyzed by 14-plex high sensitivity T-cell cytokine array. With increasing concentrations of tacrolimus, there was a trend to reduction in the release of IL-2 (p = .0137), and IFN-γ (p = .0147) in response to peptide stimulation. There was al"],"journal":["Transplant infectious disease : an official journal of the Transplantation Society"],"pagination":["e13743"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8646571"],"repository":["biostudies-literature"],"pubmed_title":["Impact of immunosuppression on the immune response to SARS-CoV-2 infection: A mechanistic study."],"pmcid":["PMC8646571"],"pubmed_authors":["Kumar D","Hall VG","Humar A","Ferreira V"],"additional_accession":[]},"is_claimable":false,"name":"Impact of immunosuppression on the immune response to SARS-CoV-2 infection: A mechanistic study.","description":"The optimal management of immunosuppression in transplant patients infected with COVID-19 is unknown. We performed an in vitro study to determine the effect of individual immunosuppressive agents on SARS-CoV-2-specific T-cell cytokine expression. Convalescent peripheral blood mononuclear cells from eleven non-immunosuppressed patients with COVID-19 were preincubated with clinically relevant concentrations of immunosuppressive drugs (tacrolimus, mycophenolate, sirolimus, prednisone) and then stimulated with a SARS-CoV-2 peptide pool. Supernatants were analyzed by 14-plex high sensitivity T-cell cytokine array. With increasing concentrations of tacrolimus, there was a trend to reduction in the release of IL-2 (p = .0137), and IFN-γ (p = .0147) in response to peptide stimulation. There was al","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T09:42:05.658Z","creation":"2022-02-11T13:49:07.32Z"},"accession":"S-EPMC8646571","cross_references":{"pubmed":["34668283"],"doi":["10.1111/tid.13743"]}}