biostudies-literatureUnknown23(6)Hall VGthe Ajmera Transplant CentreThe optimal management of immunosuppression in transplant patients infected with COVID-19 is unknown. We performed an in vitro study to determine the effect of individual immunosuppressive agents on SARS-CoV-2-specific T-cell cytokine expression. Convalescent peripheral blood mononuclear cells from eleven non-immunosuppressed patients with COVID-19 were preincubated with clinically relevant concentrations of immunosuppressive drugs (tacrolimus, mycophenolate, sirolimus, prednisone) and then stimulated with a SARS-CoV-2 peptide pool. Supernatants were analyzed by 14-plex high sensitivity T-cell cytokine array. With increasing concentrations of tacrolimus, there was a trend to reduction in the release of IL-2 (p = .0137), and IFN-γ (p = .0147) in response to peptide stimulation. There was alTransplant infectious disease : an official journal of the Transplantation Societye13743https://www.ebi.ac.uk/biostudies/studies/S-EPMC8646571biostudies-literatureImpact of immunosuppression on the immune response to SARS-CoV-2 infection: A mechanistic study.PMC8646571Kumar DHall VGHumar AFerreira VfalseImpact of immunosuppression on the immune response to SARS-CoV-2 infection: A mechanistic study.The optimal management of immunosuppression in transplant patients infected with COVID-19 is unknown. We performed an in vitro study to determine the effect of individual immunosuppressive agents on SARS-CoV-2-specific T-cell cytokine expression. Convalescent peripheral blood mononuclear cells from eleven non-immunosuppressed patients with COVID-19 were preincubated with clinically relevant concentrations of immunosuppressive drugs (tacrolimus, mycophenolate, sirolimus, prednisone) and then stimulated with a SARS-CoV-2 peptide pool. Supernatants were analyzed by 14-plex high sensitivity T-cell cytokine array. With increasing concentrations of tacrolimus, there was a trend to reduction in the release of IL-2 (p = .0137), and IFN-γ (p = .0147) in response to peptide stimulation. There was al2021-01-01T00:00:00Z2021 Dec2025-04-04T09:42:05.658Z2022-02-11T13:49:07.32ZS-EPMC86465713466828310.1111/tid.13743