biostudies-literatureKozminsky MUniversity of California, BerkeleyFred Hutchinson Cancer Research CenterNIH/NCICITRIS COVID 19 Response Seed FundingUniversity of California BerkeleyNCI NIH HHSMichael and Margaret CheccaUniversity of California Cancer Research Coordinating Committee Fellowshipe2101166https://www.ebi.ac.uk/biostudies/studies/S-EPMC8646752biostudies-literatureUnknown8(23)Lipid-based nanoparticles have been applied extensively in drug delivery and vaccine strategies and are finding diverse applications in the coronavirus disease 2019 (COVID-19) pandemic-from vaccine-component encapsulation to modeling the virus, itself. High-throughput, highly flexible methods for characterization are of great benefit to the development of liposomes featuring surface proteins. DNA-directed patterning is one such method that offers versatility in immobilizing and segregating lipid-based nanoparticles for subsequent analysis. Here, oligonucleotides are selectively conjugated onto a glass substrate and then hybridized to complementary oligonucleotides tagged to liposomes, patterning them with great control and precision. The power of DNA-directed patterning is demonstrated by Advanced science (Weinheim, Baden-Wurttemberg, Germany)DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2.PMC8646752F32 CA2433541F32CA243354-01Sohn LLCarey TRKozminsky MfalseDNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2.Lipid-based nanoparticles have been applied extensively in drug delivery and vaccine strategies and are finding diverse applications in the coronavirus disease 2019 (COVID-19) pandemic-from vaccine-component encapsulation to modeling the virus, itself. High-throughput, highly flexible methods for characterization are of great benefit to the development of liposomes featuring surface proteins. DNA-directed patterning is one such method that offers versatility in immobilizing and segregating lipid-based nanoparticles for subsequent analysis. Here, oligonucleotides are selectively conjugated onto a glass substrate and then hybridized to complementary oligonucleotides tagged to liposomes, patterning them with great control and precision. The power of DNA-directed patterning is demonstrated by 2021-01-01T00:00:00Z2021 Dec2026-05-08T00:05:41.157Z2022-02-11T14:17:26.638ZS-EPMC86467523467211710.1002/advs.202101166