{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Xia Y"],"pubmed_abstract":["<b>Background:</b> Valproic acid (VPA) is a widely used antiseizure medication and its dosing needs to be tailored individually through therapeutic drug monitoring (TDM) to avoid or prevent toxicity. Currently, immune-enzymatic assays such as Enzyme Multiplied Immunoassay Technique (EMIT), and Liquid Chromatography (LC)-based techniques, particularly coupled to Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS), resulting a potential lack of concordance between laboratories. <b>Methods:</b> In this study, plasma VPA concentrations were determined for 711 pediatric patients with epilepsy by a routine EMIT assay and by a validated in-house LC-ESI-MS/MS method on the same group of samples, aimed to address the aforementioned concern. Consistency between two assays was evaluated u"],"journal":["Frontiers in pharmacology"],"pagination":["750744"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8650496"],"repository":["biostudies-literature"],"pubmed_title":["Switching Between LC-ESI-MS/MS and EMIT Methods for Routine TDM of Valproic Acid in Pediatric Patients With Epilepsy: What Clinicians and Researchers Need to Know."],"pmcid":["PMC8650496"],"pubmed_authors":["Xia Y","Shen MY","Long JY","Guo HL","Chen F","Qiu JC","Dong N","Lu XP","Hu YH","Ding XS"],"additional_accession":[]},"is_claimable":false,"name":"Switching Between LC-ESI-MS/MS and EMIT Methods for Routine TDM of Valproic Acid in Pediatric Patients With Epilepsy: What Clinicians and Researchers Need to Know.","description":"<b>Background:</b> Valproic acid (VPA) is a widely used antiseizure medication and its dosing needs to be tailored individually through therapeutic drug monitoring (TDM) to avoid or prevent toxicity. Currently, immune-enzymatic assays such as Enzyme Multiplied Immunoassay Technique (EMIT), and Liquid Chromatography (LC)-based techniques, particularly coupled to Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS), resulting a potential lack of concordance between laboratories. <b>Methods:</b> In this study, plasma VPA concentrations were determined for 711 pediatric patients with epilepsy by a routine EMIT assay and by a validated in-house LC-ESI-MS/MS method on the same group of samples, aimed to address the aforementioned concern. Consistency between two assays was evaluated u","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-08T05:02:06.703Z","creation":"2025-06-01T02:42:58.64Z"},"accession":"S-EPMC8650496","cross_references":{"pubmed":["34887756"],"doi":["10.3389/fphar.2021.750744"]}}