{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li L"],"funding":["Harry T. Mangurian Jr. Foundation","NCI NIH HHS","National Institutes of Health","Elsa U. Pardee Foundation","Florida Department of Health","NIGMS NIH HHS","Leukemia and Lymphoma Society"],"pagination":["1595-1609"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8653786"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(23-24)"],"pubmed_abstract":["Binding of microRNAs (miRNAs) to mRNAs normally results in post-transcriptional repression of gene expression. However, extensive base-pairing between miRNAs and target RNAs can trigger miRNA degradation, a phenomenon called target RNA-directed miRNA degradation (TDMD). Here, we systematically analyzed Argonaute-CLASH (cross-linking, ligation, and sequencing of miRNA-target RNA hybrids) data and identified numerous candidate TDMD triggers, focusing on their ability to induce nontemplated nucleotide addition at the miRNA 3' end. When exogenously expressed in various cell lines, eight triggers induce degradation of corresponding miRNAs. Both the TDMD base-pairing and surrounding sequences are essential for TDMD. CRISPR knockout of endogenous trigger or ZSWIM8, a ubiquitin ligase essential fo"],"journal":["Genes & development"],"pubmed_title":["Widespread microRNA degradation elements in target mRNAs can assist the encoded proteins."],"pmcid":["PMC8653786"],"funding_grant_id":["R35GM128753","T32CA257923","R35 GM128753","R01 CA195732","R01CA195732","21L03","3399-20","T32 CA257923"],"pubmed_authors":["Li J","Li T","Fields CJ","Xie M","Sheng P","Guardia CM","Wang Y","Li L","Hiers NM","Licht JD"],"additional_accession":[]},"is_claimable":false,"name":"Widespread microRNA degradation elements in target mRNAs can assist the encoded proteins.","description":"Binding of microRNAs (miRNAs) to mRNAs normally results in post-transcriptional repression of gene expression. However, extensive base-pairing between miRNAs and target RNAs can trigger miRNA degradation, a phenomenon called target RNA-directed miRNA degradation (TDMD). Here, we systematically analyzed Argonaute-CLASH (cross-linking, ligation, and sequencing of miRNA-target RNA hybrids) data and identified numerous candidate TDMD triggers, focusing on their ability to induce nontemplated nucleotide addition at the miRNA 3' end. When exogenously expressed in various cell lines, eight triggers induce degradation of corresponding miRNAs. Both the TDMD base-pairing and surrounding sequences are essential for TDMD. CRISPR knockout of endogenous trigger or ZSWIM8, a ubiquitin ligase essential fo","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2026-05-09T20:13:24.969Z","creation":"2025-02-19T00:56:25.024Z"},"accession":"S-EPMC8653786","cross_references":{"pubmed":["34819352"],"doi":["10.1101/gad.348874.121"]}}