{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liang D"],"funding":["Charlotte Research Institute, University of North Carolina at Charlotte","NIDDK NIH HHS","Alfred P Sloan Foundation","National Institutes of Health","Cancer Research Institute","Cornell University","National Science Foundation Division of Chemistry","Arnold and Mabel Beckman Foundation","Chulabhorn Research Institute","National Science Foundation"],"pagination":["67-73.e3"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8655016"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(1)"],"pubmed_abstract":["Canonically, G-protein-coupled receptor (GPCR) signaling is transient and confined to the plasma membrane (PM). Deviating from this paradigm, the parathyroid hormone receptor (PTHR1) stimulates sustained G<sub>s</sub> signaling at endosomes. In addition to G<sub>s</sub>, PTHR1 activates G<sub>q</sub> signaling; yet, in contrast to the PTHR1-G<sub>s</sub> pathway, the spatiotemporal dynamics of the G<sub>q</sub> branch of PTHR1 signaling and its relationship to G<sub>s</sub> signaling remain largely ill defined. Recognizing that a downstream consequence of G<sub>q</sub> signaling is the activation of phospholipase D (PLD) enzymes, we leverage activity-based, bioorthogonal imaging tools for PLD signaling to visualize and quantify the G<sub>q</sub> branch of PTHR1 signaling. We establish that"],"journal":["Cell chemical biology"],"pubmed_title":["Activity-based, bioorthogonal imaging of phospholipase D reveals spatiotemporal dynamics of GPCR-Gq signaling."],"pmcid":["PMC8655016"],"funding_grant_id":["P01DK011794","CHE-1749919","P01 DK011794"],"pubmed_authors":["Liang D","Gardella TJ","Watanabe T","Cheloha RW","Baskin JM"],"additional_accession":[]},"is_claimable":false,"name":"Activity-based, bioorthogonal imaging of phospholipase D reveals spatiotemporal dynamics of GPCR-Gq signaling.","description":"Canonically, G-protein-coupled receptor (GPCR) signaling is transient and confined to the plasma membrane (PM). Deviating from this paradigm, the parathyroid hormone receptor (PTHR1) stimulates sustained G<sub>s</sub> signaling at endosomes. In addition to G<sub>s</sub>, PTHR1 activates G<sub>q</sub> signaling; yet, in contrast to the PTHR1-G<sub>s</sub> pathway, the spatiotemporal dynamics of the G<sub>q</sub> branch of PTHR1 signaling and its relationship to G<sub>s</sub> signaling remain largely ill defined. Recognizing that a downstream consequence of G<sub>q</sub> signaling is the activation of phospholipase D (PLD) enzymes, we leverage activity-based, bioorthogonal imaging tools for PLD signaling to visualize and quantify the G<sub>q</sub> branch of PTHR1 signaling. We establish that","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-04T13:40:38.791Z","creation":"2024-11-09T02:23:06.714Z"},"accession":"S-EPMC8655016","cross_references":{"pubmed":["34161786"],"doi":["10.1016/j.chembiol.2021.05.020"]}}