{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(14)"],"submitter":["Gao H"],"pubmed_abstract":["<h4>Purpose</h4>The purpose of this study was to understand the impact of Demodex infection in the lipid component of meibum in patients.<h4>Methods</h4>The meibum samples were collected from four groups of subjects: (1) Demodex-negative with non-MGD (D-M-; n = 10); (2) Demodex-positive with non-MGD (D+M-; n = 10); (3) Demodex-negative with MGD (D-M+; n = 10); and (4) Demodex-positive with MGD (D+M+; n = 10). A liquid chromatography-mass spectrometry (LC-MS) system consisting of ultra-performance liquid chromatography and a Q Exactive high-resolution mass spectrometer was used for lipids separation and detection.<h4>Results</h4>Compared with the D-M- group, the D+M- group had lower levels of phosphatidylcholines (PCs) and lysophosphatidylcholines (LPCs) and higher levels of phosphatidylethanolamines (PEs). Compared with the D-M+ group, the levels of sphingomyelins (SMs) and PCs in the D+M+ group were decreased, whereas the levels of (O-acyl)-ω-hydroxy fatty acids (OAHFAs), ceramides (CERs), LPCs, and diacylglycerols (DGs) were significantly increased. Triacylglycerols (TGs), DGs, CERs, and OAHFAs were decreased in D-M+ group, whereas levels of PEs, phosphatidylinositols, and phosphatidylglycerols were increased in meibum obtained from the D-M+ group compared with those in the D-M- group. TGs, SMs, CERs, and PEs were decreased in the D+M+ group, whereas levels of LPCs, LPEs, PCs, and PEs were increased in meibum from the D-M+ group compared with those in the D+M- group.<h4>Conclusions</h4>To the best of our knowledge, this is the first study to assess the changes in meibum from patients with ocular Demodex infestation. The significant increase of OAHFAs in the Demodex-positive group suggest that OAHFAs may be associated with the progress of ocular Demodex infections.<h4>Translational relevance</h4>OAHFAs could be a potential new therapeutic target for ocular Demodex infestation."],"journal":["Translational vision science & technology"],"pagination":["6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8662575"],"repository":["biostudies-literature"],"pubmed_title":["Changes in Meibum Lipid Composition With Ocular Demodex Infestation."],"pmcid":["PMC8662575"],"pubmed_authors":["Huang YK","Xie HT","Shi BJ","Gao H","Chen H","Xu KK"],"additional_accession":[]},"is_claimable":false,"name":"Changes in Meibum Lipid Composition With Ocular Demodex Infestation.","description":"<h4>Purpose</h4>The purpose of this study was to understand the impact of Demodex infection in the lipid component of meibum in patients.<h4>Methods</h4>The meibum samples were collected from four groups of subjects: (1) Demodex-negative with non-MGD (D-M-; n = 10); (2) Demodex-positive with non-MGD (D+M-; n = 10); (3) Demodex-negative with MGD (D-M+; n = 10); and (4) Demodex-positive with MGD (D+M+; n = 10). A liquid chromatography-mass spectrometry (LC-MS) system consisting of ultra-performance liquid chromatography and a Q Exactive high-resolution mass spectrometer was used for lipids separation and detection.<h4>Results</h4>Compared with the D-M- group, the D+M- group had lower levels of phosphatidylcholines (PCs) and lysophosphatidylcholines (LPCs) and higher levels of phosphatidylethanolamines (PEs). Compared with the D-M+ group, the levels of sphingomyelins (SMs) and PCs in the D+M+ group were decreased, whereas the levels of (O-acyl)-ω-hydroxy fatty acids (OAHFAs), ceramides (CERs), LPCs, and diacylglycerols (DGs) were significantly increased. Triacylglycerols (TGs), DGs, CERs, and OAHFAs were decreased in D-M+ group, whereas levels of PEs, phosphatidylinositols, and phosphatidylglycerols were increased in meibum obtained from the D-M+ group compared with those in the D-M- group. TGs, SMs, CERs, and PEs were decreased in the D+M+ group, whereas levels of LPCs, LPEs, PCs, and PEs were increased in meibum from the D-M+ group compared with those in the D+M- group.<h4>Conclusions</h4>To the best of our knowledge, this is the first study to assess the changes in meibum from patients with ocular Demodex infestation. The significant increase of OAHFAs in the Demodex-positive group suggest that OAHFAs may be associated with the progress of ocular Demodex infections.<h4>Translational relevance</h4>OAHFAs could be a potential new therapeutic target for ocular Demodex infestation.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T08:41:13.85Z","creation":"2025-04-04T08:41:13.85Z"},"accession":"S-EPMC8662575","cross_references":{"pubmed":["34874449"],"doi":["10.1167/tvst.10.14.6"]}}