{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["38(12)"],"submitter":["Pan M"],"pubmed_abstract":["<h4>Purpose</h4>This study was conducted to verify if the cfDNA integrity (cfDI) in follicular fluid and subsequent spent embryo medium (SEM) could serve as potential non-invasive biomarker for high-grade embryo selection during IVF/ICSI.<h4>Methods</h4>Thirty-two follicular fluids, 32 subsequent corresponding cleavage embryo SEM, and 23 subsequent blastocyst SEM were collected from 11 patients undergoing IVF/ICSI. CfDI was measured by ALU gene amplicons with different sizes by qPCR, as the ratio of long to short fragments.<h4>Results</h4>CfDI in follicular fluid corresponding to subsequent high-grade cleavage embryos and blastocysts was significantly lower than that related to low-grade embryos (p = 0.018). Conversely, cfDI in SEM was significantly and positively correlated with high-grad"],"journal":["Journal of assisted reproduction and genetics"],"pagination":["3113-3124"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8666408"],"repository":["biostudies-literature"],"pubmed_title":["The integrity of cfDNA in follicular fluid and spent medium from embryo culture is associated with embryo grade in patients undergoing in vitro fertilization."],"pmcid":["PMC8666408"],"pubmed_authors":["Liu Z","Dong J","Cai L","Shi H","Ge Q","Pan M"],"additional_accession":[]},"is_claimable":false,"name":"The integrity of cfDNA in follicular fluid and spent medium from embryo culture is associated with embryo grade in patients undergoing in vitro fertilization.","description":"<h4>Purpose</h4>This study was conducted to verify if the cfDNA integrity (cfDI) in follicular fluid and subsequent spent embryo medium (SEM) could serve as potential non-invasive biomarker for high-grade embryo selection during IVF/ICSI.<h4>Methods</h4>Thirty-two follicular fluids, 32 subsequent corresponding cleavage embryo SEM, and 23 subsequent blastocyst SEM were collected from 11 patients undergoing IVF/ICSI. CfDI was measured by ALU gene amplicons with different sizes by qPCR, as the ratio of long to short fragments.<h4>Results</h4>CfDI in follicular fluid corresponding to subsequent high-grade cleavage embryos and blastocysts was significantly lower than that related to low-grade embryos (p = 0.018). Conversely, cfDI in SEM was significantly and positively correlated with high-grad","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-19T18:55:16.29Z","creation":"2025-04-19T18:55:16.29Z"},"accession":"S-EPMC8666408","cross_references":{"pubmed":["34820723"],"doi":["10.1007/s10815-021-02357-0"]}}