{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(22)"],"submitter":["Huang M"],"pubmed_abstract":["<h4>Background</h4>The growth hormone inhibitor somatostatin and its analogs are promising therapeutic agents for acute pancreatitis. However, the therapeutic effects remain controversial. Somatostatin analogs preferentially bind to somatostatin receptor 2 (SSTR2), and this study aimed to investigate whether N-glycosylation affects SSTR2 stability, membrane trafficking, and signal transduction.<h4>Methods</h4>Western blot analysis following PNGase F digestion was performed to confirm N-glycosylation of SSTR2 in rat pancreatic acinar AR42J cells. Rats were subjected to 4 hourly intraperitoneal injections of cerulein (50 µg/kg) plus LPS (5 µg/mL) to induce acute pancreatitis. Mass spectrometry was conducted to identify the glycosylation sites of SSTR2, and immunofluorescent staining was carr"],"journal":["Annals of translational medicine"],"pagination":["1667"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8667150"],"repository":["biostudies-literature"],"pubmed_title":["N-glycosylation of somatostatin receptor type 2 protects rats from acute pancreatitis."],"pmcid":["PMC8667150"],"pubmed_authors":["Huang M","Chen W","Chen MM","Xu F","Han D"],"additional_accession":[]},"is_claimable":false,"name":"N-glycosylation of somatostatin receptor type 2 protects rats from acute pancreatitis.","description":"<h4>Background</h4>The growth hormone inhibitor somatostatin and its analogs are promising therapeutic agents for acute pancreatitis. However, the therapeutic effects remain controversial. Somatostatin analogs preferentially bind to somatostatin receptor 2 (SSTR2), and this study aimed to investigate whether N-glycosylation affects SSTR2 stability, membrane trafficking, and signal transduction.<h4>Methods</h4>Western blot analysis following PNGase F digestion was performed to confirm N-glycosylation of SSTR2 in rat pancreatic acinar AR42J cells. Rats were subjected to 4 hourly intraperitoneal injections of cerulein (50 µg/kg) plus LPS (5 µg/mL) to induce acute pancreatitis. Mass spectrometry was conducted to identify the glycosylation sites of SSTR2, and immunofluorescent staining was carr","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-06-01T03:23:15.438Z","creation":"2025-06-01T03:23:15.438Z"},"accession":"S-EPMC8667150","cross_references":{"pubmed":["34988176"],"doi":["10.21037/atm-21-5249"]}}