biostudies-literatureUnknown9(22)Huang M<h4>Background</h4>The growth hormone inhibitor somatostatin and its analogs are promising therapeutic agents for acute pancreatitis. However, the therapeutic effects remain controversial. Somatostatin analogs preferentially bind to somatostatin receptor 2 (SSTR2), and this study aimed to investigate whether N-glycosylation affects SSTR2 stability, membrane trafficking, and signal transduction.<h4>Methods</h4>Western blot analysis following PNGase F digestion was performed to confirm N-glycosylation of SSTR2 in rat pancreatic acinar AR42J cells. Rats were subjected to 4 hourly intraperitoneal injections of cerulein (50 µg/kg) plus LPS (5 µg/mL) to induce acute pancreatitis. Mass spectrometry was conducted to identify the glycosylation sites of SSTR2, and immunofluorescent staining was carrAnnals of translational medicine1667https://www.ebi.ac.uk/biostudies/studies/S-EPMC8667150biostudies-literatureN-glycosylation of somatostatin receptor type 2 protects rats from acute pancreatitis.PMC8667150Huang MChen WChen MMXu FHan DfalseN-glycosylation of somatostatin receptor type 2 protects rats from acute pancreatitis.<h4>Background</h4>The growth hormone inhibitor somatostatin and its analogs are promising therapeutic agents for acute pancreatitis. However, the therapeutic effects remain controversial. Somatostatin analogs preferentially bind to somatostatin receptor 2 (SSTR2), and this study aimed to investigate whether N-glycosylation affects SSTR2 stability, membrane trafficking, and signal transduction.<h4>Methods</h4>Western blot analysis following PNGase F digestion was performed to confirm N-glycosylation of SSTR2 in rat pancreatic acinar AR42J cells. Rats were subjected to 4 hourly intraperitoneal injections of cerulein (50 µg/kg) plus LPS (5 µg/mL) to induce acute pancreatitis. Mass spectrometry was conducted to identify the glycosylation sites of SSTR2, and immunofluorescent staining was carr2021-01-01T00:00:00Z2021 Nov2025-06-01T03:23:15.438Z2025-06-01T03:23:15.438ZS-EPMC86671503498817610.21037/atm-21-5249