{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sciortino VM"],"funding":["NINDS NIH HHS"],"pagination":["3187-3199"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8669277"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["41(12)"],"pubmed_abstract":["Multi-parametric photoacoustic microscopy (PAM) has emerged as a promising new technique for high-resolution quantification of hemodynamics and oxygen metabolism in the mouse brain. In this work, we have extended the scope of multi-parametric PAM to longitudinal, cortex-wide, awake-brain imaging with the use of a long-lifetime (24 weeks), wide-field (5 × 7 mm<sup>2</sup>), light-weight (2 g), dual-transparency (<i>i.e.,</i> light and ultrasound) cranial window. Cerebrovascular responses to the window installation were examined <i>in vivo</i>, showing a complete recovery in 18 days. In the 22-week monitoring after the recovery, no dura thickening, skull regrowth, or changes in cerebrovascular structure and function were observed. The promise of this technique was demonstrated by monitoring "],"journal":["Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism"],"pubmed_title":["Longitudinal cortex-wide monitoring of cerebral hemodynamics and oxygen metabolism in awake mice using multi-parametric photoacoustic microscopy."],"pmcid":["PMC8669277"],"funding_grant_id":["R01 NS084028","R01 NS099261","R37 NS110699","R01 NS120481","R01 NS095064","R01 NS108763"],"pubmed_authors":["Sun YY","Sun N","Hu S","Yan P","Tran A","Sun T","Zhong F","Sciortino VM","Lee JM","Zhou Y","Kuan CY","Cao R"],"additional_accession":[]},"is_claimable":false,"name":"Longitudinal cortex-wide monitoring of cerebral hemodynamics and oxygen metabolism in awake mice using multi-parametric photoacoustic microscopy.","description":"Multi-parametric photoacoustic microscopy (PAM) has emerged as a promising new technique for high-resolution quantification of hemodynamics and oxygen metabolism in the mouse brain. In this work, we have extended the scope of multi-parametric PAM to longitudinal, cortex-wide, awake-brain imaging with the use of a long-lifetime (24 weeks), wide-field (5 × 7 mm<sup>2</sup>), light-weight (2 g), dual-transparency (<i>i.e.,</i> light and ultrasound) cranial window. Cerebrovascular responses to the window installation were examined <i>in vivo</i>, showing a complete recovery in 18 days. In the 22-week monitoring after the recovery, no dura thickening, skull regrowth, or changes in cerebrovascular structure and function were observed. The promise of this technique was demonstrated by monitoring ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-18T13:15:43.575Z","creation":"2025-04-06T22:49:18.85Z"},"accession":"S-EPMC8669277","cross_references":{"pubmed":["34304622"],"doi":["10.1177/0271678X211034096","10.1177/0271678x211034096"]}}