biostudies-literatureUnknown9Wilkinson HNEndogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endogenous metals for cutaneous repair remains largely unexplored. Here we combine ICP-MS with tissue-level RNA-sequencing to reveal profound changes in a number of metals, and corresponding metal-regulated genes, across temporal healing in mice. Wound calcium, magnesium, iron, copper and manganese are elevated at 7 days post-wounding, while magnesium, iron, aluminium, manganese and cobalt increase at 14 days post-wounding. At the level of transcription, wound-induced pathways are independently highFrontiers in cell and developmental biology788596https://www.ebi.ac.uk/biostudies/studies/S-EPMC8669724biostudies-literatureCombined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.PMC8669724Hardman MJWilkinson HNGuinn BAfalseCombined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.Endogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endogenous metals for cutaneous repair remains largely unexplored. Here we combine ICP-MS with tissue-level RNA-sequencing to reveal profound changes in a number of metals, and corresponding metal-regulated genes, across temporal healing in mice. Wound calcium, magnesium, iron, copper and manganese are elevated at 7 days post-wounding, while magnesium, iron, aluminium, manganese and cobalt increase at 14 days post-wounding. At the level of transcription, wound-induced pathways are independently high2021-01-01T00:00:00Z20212026-04-08T09:59:01.105Z2022-02-11T14:22:44.804ZS-EPMC86697243491762110.3389/fcell.2021.788596