{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sugiyama T"],"funding":["NIEHS NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["111762"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8671204"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["823"],"pubmed_abstract":["Although UV-induced mutagenesis has been studied extensively, the precise mechanisms that convert UV-induced DNA damage into mutations remain elusive. One well-studied mechanism involves DNA polymerase (Pol) η and ζ, which produces C > T transitions during translesion synthesis (TLS) across pyrimidine dimers. We previously proposed another biochemical mechanism that involves multiple UV-irradiations with incubation in the dark in between. The incubation facilitates spontaneous deamination of cytosine in a pyrimidine dimer, and the subsequent UV irradiation induces photolyase-independent (direct) photoreversal that converts cytosine into monomeric uracil residue. In this paper, we first demonstrate that natural sunlight can induce both mutational processes in vitro. The direct photoreversal"],"journal":["Mutation research"],"pubmed_title":["Biochemical and photochemical mechanisms that produce different UV-induced mutation spectra."],"pmcid":["PMC8671204"],"funding_grant_id":["R15ES029723","R15 ES029723","R15GM116098","R15 GM116098"],"pubmed_authors":["Sanyal MR","Keinard B","Sugiyama T","Best G"],"additional_accession":[]},"is_claimable":false,"name":"Biochemical and photochemical mechanisms that produce different UV-induced mutation spectra.","description":"Although UV-induced mutagenesis has been studied extensively, the precise mechanisms that convert UV-induced DNA damage into mutations remain elusive. One well-studied mechanism involves DNA polymerase (Pol) η and ζ, which produces C > T transitions during translesion synthesis (TLS) across pyrimidine dimers. We previously proposed another biochemical mechanism that involves multiple UV-irradiations with incubation in the dark in between. The incubation facilitates spontaneous deamination of cytosine in a pyrimidine dimer, and the subsequent UV irradiation induces photolyase-independent (direct) photoreversal that converts cytosine into monomeric uracil residue. In this paper, we first demonstrate that natural sunlight can induce both mutational processes in vitro. The direct photoreversal","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul-Dec","modification":"2025-04-05T16:10:17.907Z","creation":"2025-02-19T00:47:39.383Z"},"accession":"S-EPMC8671204","cross_references":{"pubmed":["34563793"],"doi":["10.1016/j.mrfmmm.2021.111762"]}}