biostudies-literatureKhaing KKUniversität für Bodenkultur WienThailand Science Research and InnovationSuranaree University of TechnologyNational Research Council of Thailande0209421https://www.ebi.ac.uk/biostudies/studies/S-EPMC8672896biostudies-literatureUnknown9(3)<i>Bradyrhizobium</i> is an endophytic bacterium under investigation as an efficient biofertilizer for sustainable legume-rice rotational cropping system. Monitoring and bio-imaging of this nitrogen fixing bacterium is essential for the study of plant-microbe evolution, soil microbiome, as well as quality control in organic farming. While phage display antibody technology has been widely used to generate recombinant antibody for myriad medical purposes, so far, this technology has been minimally applied in the agricultural sector. In this study, single-chain variable fragments (scFv) against two <i>Bradyrhizobium</i> strains SUTN9-2 (yiN92-1e10) and DOA9 (yiDOA9-162) were isolated from a human phage display antibody library. Specific binding of scFv was demonstrated by ELISA and confocal-iMicrobiology spectrumApplication of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume.PMC867289661/13/2561NRCT 808/2563Student Exchange ProgramRTA6180012Boonkerd NYamabhai MRangnoi KMichlits HTeaumroong NTittabutr PKhaing KKfalseApplication of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume.<i>Bradyrhizobium</i> is an endophytic bacterium under investigation as an efficient biofertilizer for sustainable legume-rice rotational cropping system. Monitoring and bio-imaging of this nitrogen fixing bacterium is essential for the study of plant-microbe evolution, soil microbiome, as well as quality control in organic farming. While phage display antibody technology has been widely used to generate recombinant antibody for myriad medical purposes, so far, this technology has been minimally applied in the agricultural sector. In this study, single-chain variable fragments (scFv) against two <i>Bradyrhizobium</i> strains SUTN9-2 (yiN92-1e10) and DOA9 (yiDOA9-162) were isolated from a human phage display antibody library. Specific binding of scFv was demonstrated by ELISA and confocal-i2021-01-01T00:00:00Z2021 Dec2026-06-17T06:19:07.336Z2022-02-11T16:17:53.731ZS-EPMC86728963490845110.1128/Spectrum.02094-21