biostudies-literatureUnknown6(49)Nasir ZThe present study used a sol-gel auto-combustion approach to make silica (SiO₂)-coated Ni-Co ferrite nanocomposites that would be used as a platform for enzyme immobilization. Using glutaraldehyde as a coupling agent, glucose oxidase (GOx) was covalently immobilized on this magnetic substrate. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HRTEM), and fourier transform infrared spectroscopy (FTIR) was used to determine the structural analysis and morphology of Ni-Co ferrite/SiO₂ nanocomposites. FTIR spectra confirmed the binding of GOx to Ni-Co ferrite/SiO₂ nanocomposites, with a loading efficiency of around 85%. At alkaline pH and higher temperature,ACS omega33554-33567https://www.ebi.ac.uk/biostudies/studies/S-EPMC8675013biostudies-literatureImmobilization of GOx Enzyme on SiO₂-Coated Ni-Co Ferrite Nanocomposites as Magnetic Support and Their Antimicrobial and Photocatalytic Activities.PMC8675013Nasir ZAli ANusrat Jahan SAlam MFShoeb MfalseImmobilization of GOx Enzyme on SiO₂-Coated Ni-Co Ferrite Nanocomposites as Magnetic Support and Their Antimicrobial and Photocatalytic Activities.The present study used a sol-gel auto-combustion approach to make silica (SiO₂)-coated Ni-Co ferrite nanocomposites that would be used as a platform for enzyme immobilization. Using glutaraldehyde as a coupling agent, glucose oxidase (GOx) was covalently immobilized on this magnetic substrate. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HRTEM), and fourier transform infrared spectroscopy (FTIR) was used to determine the structural analysis and morphology of Ni-Co ferrite/SiO₂ nanocomposites. FTIR spectra confirmed the binding of GOx to Ni-Co ferrite/SiO₂ nanocomposites, with a loading efficiency of around 85%. At alkaline pH and higher temperature,2021-01-01T00:00:00Z2021 Dec2026-05-09T06:17:51.28Z2022-02-11T14:04:30.355ZS-EPMC86750133492690410.1021/acsomega.1c04360