biostudies-literatureBao WThe Natural Science Foundation of Inner Mongolia'Young Science and Technology Talents Programme' (Leading Person) in Inner Mongolia Autonomous Region Colleges and UniversitiesThe Collaborative Innovation Centre for Water Environmental Security of Inner Mongolia Autonomous Region, ChinaNational Natural Science Foundation of China512-518https://www.ebi.ac.uk/biostudies/studies/S-EPMC8675809biostudies-literatureUnknown15(5)Silver nanomaterials have become important research topics in recent years. As a new type of fluorescent material, silver nanomaterials have been applied to fluorescent sensors, bioimaging and materials targeting cancer cells. Here, an approach to the oligonucleotide-templated controllable formation of fluorescent Ag nanomaterials is reported. In this experiment, silver nanoparticles (NPs) were synthesised from oligonucleotides chains, sodium borohydride (NaBH₄ ) and silver nitrate (AgNO₃ ) by changing the molar ratio of DNA to sodium borohydride (NaBH₄ ) and silver nitrate (AgNO₃ ). Fluorescent assay and transmission electron microscopy were used to characterise the silver NPs. The optimal selection of DNA chains with different lengths as templates for the synthesis of silver NPs was found. This work successfully develops the capping oligonucleotides scaffolds of silver nanoclusters.IET nanobiotechnologyControllable synthesis of fluorescent silver nanoparticles with different length oligonucleotides.PMC8675809Grant No. 21864020Grant No. 2018MS02012Grant No. NJYT-19- A04Grant No. XTCX003Ga LBao WAi JfalseControllable synthesis of fluorescent silver nanoparticles with different length oligonucleotides.Silver nanomaterials have become important research topics in recent years. As a new type of fluorescent material, silver nanomaterials have been applied to fluorescent sensors, bioimaging and materials targeting cancer cells. Here, an approach to the oligonucleotide-templated controllable formation of fluorescent Ag nanomaterials is reported. In this experiment, silver nanoparticles (NPs) were synthesised from oligonucleotides chains, sodium borohydride (NaBH₄ ) and silver nitrate (AgNO₃ ) by changing the molar ratio of DNA to sodium borohydride (NaBH₄ ) and silver nitrate (AgNO₃ ). Fluorescent assay and transmission electron microscopy were used to characterise the silver NPs. The optimal selection of DNA chains with different lengths as templates for the synthesis of silver NPs was found. This work successfully develops the capping oligonucleotides scaffolds of silver nanoclusters.2021-01-01T00:00:00Z2021 Jul2025-04-04T08:34:34.202Z2025-04-04T08:34:34.202ZS-EPMC86758093469475710.1049/nbt2.12049