biostudies-literatureUnknown12Lu SAs alternatives to antibiotics in feed, probiotic <i>Bacillus</i> carries multiple advantages in animal production. Spores undergo strain-related germination in the gastrointestinal tract, but it is still unknown whether the probiotic function of the <i>Bacillus</i> depends on the germination of spores <i>in vivo</i>. In this study, based on 14 potential probiotic <i>Bacillus</i> strains from fermented food and feed, we detected the germination response of these <i>Bacillus</i> spores in relation to different germinating agents. The results showed the germination response was strain-specific and germinant-related, and nutrient germinant L-alanine significantly promoted the growth of strains with germination potential. Two strains of <i>Bacillus subtilis</i>, S-2 and 312, with or without a Frontiers in microbiology796158https://www.ebi.ac.uk/biostudies/studies/S-EPMC8675871biostudies-literatureNutrient L-Alanine-Induced Germination of <i>Bacillus</i> Improves Proliferation of Spores and Exerts Probiotic Effects <i>in vitro</i> and <i>in vivo</i>.PMC8675871Fang YLiao XGuo XLu SZhang LXiang MfalseNutrient L-Alanine-Induced Germination of <i>Bacillus</i> Improves Proliferation of Spores and Exerts Probiotic Effects <i>in vitro</i> and <i>in vivo</i>.As alternatives to antibiotics in feed, probiotic <i>Bacillus</i> carries multiple advantages in animal production. Spores undergo strain-related germination in the gastrointestinal tract, but it is still unknown whether the probiotic function of the <i>Bacillus</i> depends on the germination of spores <i>in vivo</i>. In this study, based on 14 potential probiotic <i>Bacillus</i> strains from fermented food and feed, we detected the germination response of these <i>Bacillus</i> spores in relation to different germinating agents. The results showed the germination response was strain-specific and germinant-related, and nutrient germinant L-alanine significantly promoted the growth of strains with germination potential. Two strains of <i>Bacillus subtilis</i>, S-2 and 312, with or without a 2021-01-01T00:00:00Z20212026-03-16T16:02:48.382Z2025-06-01T03:45:54.199ZS-EPMC86758713492530610.3389/fmicb.2021.796158