biostudies-literatureYang WNIDDK NIH HHSNIAID NIH HHSNational Institutes of Health150-165https://www.ebi.ac.uk/biostudies/studies/S-EPMC8678294biostudies-literatureUnknown162(1)<h4>Background & aims</h4>G protein-coupled receptor (GPR) 120 has been implicated in regulating metabolic syndromes with anti-inflammatory function. However, the role of GPR120 in intestinal inflammation is unknown. Here, we investigated whether and how GPR120 regulates CD4⁺ T cell function to inhibit colitis development.<h4>Methods</h4>Dextran sodium sulfate (DSS)-induced colitis model, Citrobacter rodentium infection model, and CD4⁺ T cell adoptive transfer model were used to analyze the role of GPR120 in regulating colitis development. The effect of GPR120 on CD4⁺ T cell functions was analyzed by RNA sequencing, flow cytometry, and Seahorse metabolic assays. Mice were administered GPR120 agonist for investigating the potential of GPR120 agonist in preveGastroenterologyGPR120 Inhibits Colitis Through Regulation of CD4⁺ T Cell Interleukin 10 Production.PMC8678294R21 AI150210R01 DK125011R01 DK124132Liu HXu LZhang HZuo XYao SBarnes SZhao XYang WLi YDann SMZhao QYu TCong YCohn SMfalseGPR120 Inhibits Colitis Through Regulation of CD4⁺ T Cell Interleukin 10 Production.<h4>Background & aims</h4>G protein-coupled receptor (GPR) 120 has been implicated in regulating metabolic syndromes with anti-inflammatory function. However, the role of GPR120 in intestinal inflammation is unknown. Here, we investigated whether and how GPR120 regulates CD4⁺ T cell function to inhibit colitis development.<h4>Methods</h4>Dextran sodium sulfate (DSS)-induced colitis model, Citrobacter rodentium infection model, and CD4⁺ T cell adoptive transfer model were used to analyze the role of GPR120 in regulating colitis development. The effect of GPR120 on CD4⁺ T cell functions was analyzed by RNA sequencing, flow cytometry, and Seahorse metabolic assays. Mice were administered GPR120 agonist for investigating the potential of GPR120 agonist in preve2022-01-01T00:00:00Z2022 Jan2025-04-21T14:26:58.01Z2025-02-18T22:34:23.376ZS-EPMC86782943453645110.1053/j.gastro.2021.09.018