biostudies-literatureZhang Mnational natural science foundation of chinajiangsu province’s key discipline of medicinethe priority academic program development of jiangsu higher education institutions116https://www.ebi.ac.uk/biostudies/studies/S-EPMC8684143biostudies-literatureUnknown23(1)<h4>Background</h4>Triple-negative breast cancer (TNBC) is highly metastatic and lethal. Due to a lack of druggable targets for this disease, there are no effective therapies in the clinic.<h4>Methods</h4>We used TNBC cells and xenografted mice as models to explore triptonide-mediated inhibition of TNBC metastasis and tumor growth. Colony formation assay was used to quantify the tumorigenesis of TNBC cells. Wound-healing and cell trans-well assays were utilized to measure cell migration and invasion. Tube formation assay was applied to access tumor cell-mediated vasculogenic mimicry. Western blot, quantitative-PCR, immunofluorescence imaging, and immunohistochemical staining were used to measure the expression levels of various tumorigenic genes in TNBC cells.<h4>Results</h4>Here, we showeBreast cancer research : BCRTriptonide effectively inhibits triple-negative breast cancer metastasis through concurrent degradation of Twist1 and Notch1 oncoproteins.PMC8684143XK201118No.81902647No.82073225No. 81703595No. 81772535Liu YQiao YQi JHu YLu WXu PZhou QMeng MZhang MZhao ZZhou ZfalseTriptonide effectively inhibits triple-negative breast cancer metastasis through concurrent degradation of Twist1 and Notch1 oncoproteins.<h4>Background</h4>Triple-negative breast cancer (TNBC) is highly metastatic and lethal. Due to a lack of druggable targets for this disease, there are no effective therapies in the clinic.<h4>Methods</h4>We used TNBC cells and xenografted mice as models to explore triptonide-mediated inhibition of TNBC metastasis and tumor growth. Colony formation assay was used to quantify the tumorigenesis of TNBC cells. Wound-healing and cell trans-well assays were utilized to measure cell migration and invasion. Tube formation assay was applied to access tumor cell-mediated vasculogenic mimicry. Western blot, quantitative-PCR, immunofluorescence imaging, and immunohistochemical staining were used to measure the expression levels of various tumorigenic genes in TNBC cells.<h4>Results</h4>Here, we showe2021-01-01T00:00:00Z2021 Dec2026-05-07T21:17:34.576Z2022-02-11T14:11:13.243ZS-EPMC86841433492260210.1186/s13058-021-01488-7