biostudies-literatureXie CChina Agriculture Research System of MOF and MARAthe Fundamental Research Funds for the Central Universitiesthe National Natural Science Foundation of Chinae0317321https://www.ebi.ac.uk/biostudies/studies/S-EPMC8689567biostudies-literatureUnknown12(6)Proper protein secretion is critical for fungal development and pathogenesis. However, the potential roles of proteins involved in the early secretory pathway are largely undescribed in filamentous fungi. p24 proteins are cargo receptors that cycle between the endoplasmic reticulum (ER) and Golgi apparatus in the early secretory pathway and recruit cargo proteins to nascent vesicles. This study characterized the function of two p24 family proteins (SsEmp24 and SsErv25) in a phytopathogenic fungus, Sclerotinia sclerotiorum. Both <i>SsEmp24</i> and <i>SsErv25</i> were upregulated during the early stages of <i>S. sclerotiorum</i> infection. Δ<i>SsEmp24</i> mutant and Δ<i>SsErv25</i> mutant displayed abnormal vegetative growth and sclerotium formation, were defective in infection cushion formamBioEarly Secretory Pathway-Associated Proteins SsEmp24 and SsErv25 Are Involved in Morphogenesis and Pathogenicity in a Filamentous Phytopathogenic Fungus.PMC86895672662020ZKPY01232072372 and 31501595Shang QMo CXiao YXie JXiao XWang GJiang DXie CfalseEarly Secretory Pathway-Associated Proteins SsEmp24 and SsErv25 Are Involved in Morphogenesis and Pathogenicity in a Filamentous Phytopathogenic Fungus.Proper protein secretion is critical for fungal development and pathogenesis. However, the potential roles of proteins involved in the early secretory pathway are largely undescribed in filamentous fungi. p24 proteins are cargo receptors that cycle between the endoplasmic reticulum (ER) and Golgi apparatus in the early secretory pathway and recruit cargo proteins to nascent vesicles. This study characterized the function of two p24 family proteins (SsEmp24 and SsErv25) in a phytopathogenic fungus, Sclerotinia sclerotiorum. Both <i>SsEmp24</i> and <i>SsErv25</i> were upregulated during the early stages of <i>S. sclerotiorum</i> infection. Δ<i>SsEmp24</i> mutant and Δ<i>SsErv25</i> mutant displayed abnormal vegetative growth and sclerotium formation, were defective in infection cushion forma2021-01-01T00:00:00Z2021 Dec2026-06-12T03:23:43.177Z2024-11-06T07:13:06.701ZS-EPMC86895673493345110.1128/mBio.03173-21