{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mollaei M"],"funding":["Wageningen University and Research Centre","European Research Council"],"pagination":["708911"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8691401"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["We established a syntrophic coculture of <i>Syntrophobacter fumaroxidans</i> MPOB<sup>T</sup> (SF) and <i>Geobacter sulfurreducens</i> PCA<sup>T</sup> (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion <i>via</i> the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) <i>via</i> direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF c"],"journal":["Frontiers in microbiology"],"pubmed_title":["Proteomic Analysis of a Syntrophic Coculture of <i>Syntrophobacter fumaroxidans</i> MPOB<sup>T</sup> and <i>Geobacter sulfurreducens</i> PCA<sup>T</sup>."],"pmcid":["PMC8691401"],"funding_grant_id":["323009"],"pubmed_authors":["Stams AJM","Boeren S","Sedano-Nunez VT","Plugge CM","Mollaei M","Suarez-Diez M"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic Analysis of a Syntrophic Coculture of <i>Syntrophobacter fumaroxidans</i> MPOB<sup>T</sup> and <i>Geobacter sulfurreducens</i> PCA<sup>T</sup>.","description":"We established a syntrophic coculture of <i>Syntrophobacter fumaroxidans</i> MPOB<sup>T</sup> (SF) and <i>Geobacter sulfurreducens</i> PCA<sup>T</sup> (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion <i>via</i> the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) <i>via</i> direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF c","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-08T18:13:47.975Z","creation":"2022-02-11T14:07:57.136Z"},"accession":"S-EPMC8691401","cross_references":{"pubmed":["34950111"],"doi":["10.3389/fmicb.2021.708911"]}}