biostudies-literatureMollaei MWageningen University and Research CentreEuropean Research Council708911https://www.ebi.ac.uk/biostudies/studies/S-EPMC8691401biostudies-literatureUnknown12We established a syntrophic coculture of <i>Syntrophobacter fumaroxidans</i> MPOB^T (SF) and <i>Geobacter sulfurreducens</i> PCA^T (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion <i>via</i> the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) <i>via</i> direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF cFrontiers in microbiologyProteomic Analysis of a Syntrophic Coculture of <i>Syntrophobacter fumaroxidans</i> MPOB^T and <i>Geobacter sulfurreducens</i> PCA^T.PMC8691401323009Stams AJMBoeren SSedano-Nunez VTPlugge CMMollaei MSuarez-Diez MfalseProteomic Analysis of a Syntrophic Coculture of <i>Syntrophobacter fumaroxidans</i> MPOB^T and <i>Geobacter sulfurreducens</i> PCA^T.We established a syntrophic coculture of <i>Syntrophobacter fumaroxidans</i> MPOB^T (SF) and <i>Geobacter sulfurreducens</i> PCA^T (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion <i>via</i> the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) <i>via</i> direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF c2021-01-01T00:00:00Z20212026-05-08T18:13:47.975Z2022-02-11T14:07:57.136ZS-EPMC86914013495011110.3389/fmicb.2021.708911