{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mauduit D"],"funding":["Kom op tegen Kanker","European Research Council","KU Leuven","Fonds Wetenschappelijk Onderzoek","Stichting tegen Kanker"],"pagination":["e71735"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8691835"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["Understanding how enhancers drive cell-type specificity and efficiently identifying them is essential for the development of innovative therapeutic strategies. In melanoma, the melanocytic (MEL) and the mesenchymal-like (MES) states present themselves with different responses to therapy, making the identification of specific enhancers highly relevant. Using massively parallel reporter assays (MPRAs) in a panel of patient-derived melanoma lines (MM lines), we set to identify and decipher melanoma enhancers by first focusing on regions with state-specific H3K27 acetylation close to differentially expressed genes. An in-depth evaluation of those regions was then pursued by investigating the activity of overlapping ATAC-seq peaks along with a full tiling of the acetylated regions with 190 bp s"],"journal":["eLife"],"pubmed_title":["Analysis of long and short enhancers in melanoma cell states."],"pmcid":["PMC8691835"],"funding_grant_id":["2019-100","724226_cis-CONTROL","1S03317N","C14/18/092","12J6916N","724226"],"pubmed_authors":["Christiaens V","Minnoye L","Hulselmans G","Demeulemeester J","Mauduit D","Aerts S","de Waegeneer M","Wouters J","Taskiran II"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of long and short enhancers in melanoma cell states.","description":"Understanding how enhancers drive cell-type specificity and efficiently identifying them is essential for the development of innovative therapeutic strategies. In melanoma, the melanocytic (MEL) and the mesenchymal-like (MES) states present themselves with different responses to therapy, making the identification of specific enhancers highly relevant. Using massively parallel reporter assays (MPRAs) in a panel of patient-derived melanoma lines (MM lines), we set to identify and decipher melanoma enhancers by first focusing on regions with state-specific H3K27 acetylation close to differentially expressed genes. An in-depth evaluation of those regions was then pursued by investigating the activity of overlapping ATAC-seq peaks along with a full tiling of the acetylated regions with 190 bp s","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2026-07-09T12:26:07.467Z","creation":"2022-02-11T14:37:03.825Z"},"accession":"S-EPMC8691835","cross_references":{"pubmed":["34874265"],"doi":["10.7554/eLife.71735"]}}