biostudies-literatureMauduit DKom op tegen KankerEuropean Research CouncilKU LeuvenFonds Wetenschappelijk OnderzoekStichting tegen Kankere71735https://www.ebi.ac.uk/biostudies/studies/S-EPMC8691835biostudies-literatureUnknown10Understanding how enhancers drive cell-type specificity and efficiently identifying them is essential for the development of innovative therapeutic strategies. In melanoma, the melanocytic (MEL) and the mesenchymal-like (MES) states present themselves with different responses to therapy, making the identification of specific enhancers highly relevant. Using massively parallel reporter assays (MPRAs) in a panel of patient-derived melanoma lines (MM lines), we set to identify and decipher melanoma enhancers by first focusing on regions with state-specific H3K27 acetylation close to differentially expressed genes. An in-depth evaluation of those regions was then pursued by investigating the activity of overlapping ATAC-seq peaks along with a full tiling of the acetylated regions with 190 bp seLifeAnalysis of long and short enhancers in melanoma cell states.PMC86918352019-100724226_cis-CONTROL1S03317NC14/18/09212J6916N724226Christiaens VMinnoye LHulselmans GDemeulemeester JMauduit DAerts Sde Waegeneer MWouters JTaskiran IIfalseAnalysis of long and short enhancers in melanoma cell states.Understanding how enhancers drive cell-type specificity and efficiently identifying them is essential for the development of innovative therapeutic strategies. In melanoma, the melanocytic (MEL) and the mesenchymal-like (MES) states present themselves with different responses to therapy, making the identification of specific enhancers highly relevant. Using massively parallel reporter assays (MPRAs) in a panel of patient-derived melanoma lines (MM lines), we set to identify and decipher melanoma enhancers by first focusing on regions with state-specific H3K27 acetylation close to differentially expressed genes. An in-depth evaluation of those regions was then pursued by investigating the activity of overlapping ATAC-seq peaks along with a full tiling of the acetylated regions with 190 bp s2021-01-01T00:00:00Z2021 Dec2026-07-09T12:26:07.467Z2022-02-11T14:37:03.825ZS-EPMC86918353487426510.7554/eLife.71735