{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hovde MJ"],"funding":["National Institutes of Health","Regenerative Medicine Minnesota","Arnold and Mabel Beckman Foundation","NIGMS NIH HHS","National Science Foundation"],"pagination":["120142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8692447"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["288"],"pubmed_abstract":["<h4>Aims</h4>Determine the effect of palmitoylation on the sodium hydrogen exchanger isoform 1 (NHE1), a member of the SLC9 family.<h4>Main methods</h4>NHE1 expressed in native rat tissues or in heterologous cells was assessed for palmitoylation by acyl-biotinyl exchange (ABE) and metabolic labeling with [<sup>3</sup>H]palmitate. Cellular palmitoylation was inhibited using 2-bromopalmitate (2BP) followed by determination of NHE1 palmitoylation status, intracellular pH, stress fiber formation, and cell migration. In addition, NHE1 was activated with LPA treatment followed by determination of NHE1 palmitoylation status and LPA-induced change in intracellular pH was determined in the presence and absence of preincubation with 2BP.<h4>Key findings</h4>In this study we demonstrate for the first"],"journal":["Life sciences"],"pubmed_title":["Sodium hydrogen exchanger (NHE1) palmitoylation and potential functional regulation."],"pmcid":["PMC8692447"],"funding_grant_id":["P20 GM103442","P20 GM104360"],"pubmed_authors":["Armand A","Vaughan RA","Pitsch E","Kooiker AJ","Foster JD","Bolland DE","Provost JJ","Bakker C","Hovde MJ","Wallert MA"],"additional_accession":[]},"is_claimable":false,"name":"Sodium hydrogen exchanger (NHE1) palmitoylation and potential functional regulation.","description":"<h4>Aims</h4>Determine the effect of palmitoylation on the sodium hydrogen exchanger isoform 1 (NHE1), a member of the SLC9 family.<h4>Main methods</h4>NHE1 expressed in native rat tissues or in heterologous cells was assessed for palmitoylation by acyl-biotinyl exchange (ABE) and metabolic labeling with [<sup>3</sup>H]palmitate. Cellular palmitoylation was inhibited using 2-bromopalmitate (2BP) followed by determination of NHE1 palmitoylation status, intracellular pH, stress fiber formation, and cell migration. In addition, NHE1 was activated with LPA treatment followed by determination of NHE1 palmitoylation status and LPA-induced change in intracellular pH was determined in the presence and absence of preincubation with 2BP.<h4>Key findings</h4>In this study we demonstrate for the first","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-08T02:06:25.999Z","creation":"2025-02-18T22:34:27.699Z"},"accession":"S-EPMC8692447","cross_references":{"pubmed":["34774621"],"doi":["10.1016/j.lfs.2021.120142"]}}