biostudies-literatureLi SFundamental Research Funds for the Central UniversitiesChina University of Geosciences, BeijingNational Natural Science Foundation of China671-677https://www.ebi.ac.uk/biostudies/studies/S-EPMC8693351biostudies-literatureUnknown11(2)Electrochemical aptamer-based (E-AB) sensors provide a great opportunity towards the goal of point-of-care and wearable sensing technologies due to their good sensitivity and selectivity. Nevertheless, the output signals from this sensor class remain low when sensors are interrogated <i>via</i> square-wave voltammetry. This low signaling limits the sensor's precision for its capability to detect small changes in target concentrations. To circumvent this, we proposed here the use of a readily shrink-induced, wrinkled Au-coating polyolefin film to immobilize a greater number of DNA probes and thus improve the signaling. Specifically, wrinkled gold film exhibits a 5.5-fold increase of surface area in comparison to the unwrinkled ones. Using these substrates we fabricated a set of E-AB sensorsRSC advancesA wrinkled structure of gold film greatly improves the signaling of electrochemical aptamer-based biosensors.PMC86933512187412121801231CUG170668CUG17066521804121Li HLi SPlaxco KWKhine MSi ZLin LChang XXia FfalseA wrinkled structure of gold film greatly improves the signaling of electrochemical aptamer-based biosensors.Electrochemical aptamer-based (E-AB) sensors provide a great opportunity towards the goal of point-of-care and wearable sensing technologies due to their good sensitivity and selectivity. Nevertheless, the output signals from this sensor class remain low when sensors are interrogated <i>via</i> square-wave voltammetry. This low signaling limits the sensor's precision for its capability to detect small changes in target concentrations. To circumvent this, we proposed here the use of a readily shrink-induced, wrinkled Au-coating polyolefin film to immobilize a greater number of DNA probes and thus improve the signaling. Specifically, wrinkled gold film exhibits a 5.5-fold increase of surface area in comparison to the unwrinkled ones. Using these substrates we fabricated a set of E-AB sensors2020-01-01T00:00:00Z2020 Dec2025-04-25T22:21:41.93Z2025-04-06T09:05:10.773ZS-EPMC86933513542369310.1039/d0ra09174j