biostudies-literatureKariyawasam HChan Zuckerberg Initiative Essential Open Source Software for Science Programlqab116https://www.ebi.ac.uk/biostudies/studies/S-EPMC8693569biostudies-literatureUnknown3(4)<i>Glimma</i> 1.0 introduced intuitive, point-and-click interactive graphics for differential gene expression analysis. Here, we present a major update to <i>Glimma</i> that brings improved interactivity and reproducibility using high-level visualization frameworks for R and JavaScript. <i>Glimma</i> 2.0 plots are now readily embeddable in R Markdown, thus allowing users to create reproducible reports containing interactive graphics. The revamped multidimensional scaling plot features dashboard-style controls allowing the user to dynamically change the colour, shape and size of sample points according to different experimental conditions. Interactivity was enhanced in the MA-style plot for comparing differences to average expression, which now supports selecting multiple genes, export optiNAR genomics and bioinformaticsDashboard-style interactive plots for RNA-seq analysis are R Markdown ready with <i>Glimma</i> 2.0.PMC86935692019-207283Su SKariyawasam HVoogd ORitchie MELaw CWfalseDashboard-style interactive plots for RNA-seq analysis are R Markdown ready with <i>Glimma</i> 2.0.<i>Glimma</i> 1.0 introduced intuitive, point-and-click interactive graphics for differential gene expression analysis. Here, we present a major update to <i>Glimma</i> that brings improved interactivity and reproducibility using high-level visualization frameworks for R and JavaScript. <i>Glimma</i> 2.0 plots are now readily embeddable in R Markdown, thus allowing users to create reproducible reports containing interactive graphics. The revamped multidimensional scaling plot features dashboard-style controls allowing the user to dynamically change the colour, shape and size of sample points according to different experimental conditions. Interactivity was enhanced in the MA-style plot for comparing differences to average expression, which now supports selecting multiple genes, export opti2021-01-01T00:00:00Z2021 Dec2025-04-22T10:16:56.942Z2022-02-11T14:47:36.94ZS-EPMC86935693498843910.1093/nargab/lqab116