biostudies-literatureWang CHHigher Education Sprout Project of Ministry of Education in TaiwanWan Fang Hospital, Taipei Medical Universitye0071621https://www.ebi.ac.uk/biostudies/studies/S-EPMC8694153biostudies-literatureUnknown9(3)Studies involving the pathogenic organism Mycobacterium tuberculosis routinely require advanced biosafety laboratory facilities, which might not be readily available in rural areas where tuberculosis burdens are high. Attempts to adapt heat inactivation techniques have led to inconsistent conclusions, and the risk of protein denaturation due to extensive heating is impractical for subsequent mass spectrometry (MS)-based protein analyses. In this study, 240 specimens with one or two loops of M. tuberculosis strain H37Rv biomass and specific inactivated solutions were proportionally assigned to six heat inactivation methods in a thermal block at 80°C and 95°C for 20, 30, and 90 min. Twenty untreated specimens served as a positive control, and bacterial growth was followed up for 12 weeks. OuMicrobiology spectrumBiosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis.PMC8694153108TMU-WFH-22DP2-110-21121-01-I-07Lee JCWang CHTsai ILPutri DULee CHLiao CCTsao STHsiao ALChen XWWu JHfalseBiosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis.Studies involving the pathogenic organism Mycobacterium tuberculosis routinely require advanced biosafety laboratory facilities, which might not be readily available in rural areas where tuberculosis burdens are high. Attempts to adapt heat inactivation techniques have led to inconsistent conclusions, and the risk of protein denaturation due to extensive heating is impractical for subsequent mass spectrometry (MS)-based protein analyses. In this study, 240 specimens with one or two loops of M. tuberculosis strain H37Rv biomass and specific inactivated solutions were proportionally assigned to six heat inactivation methods in a thermal block at 80°C and 95°C for 20, 30, and 90 min. Twenty untreated specimens served as a positive control, and bacterial growth was followed up for 12 weeks. Ou2021-01-01T00:00:00Z2021 Dec2026-06-15T06:00:52.48Z2025-04-05T23:16:04.127ZS-EPMC86941533493719410.1128/spectrum.00716-21