{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(8)"],"submitter":["Tu J"],"pubmed_abstract":["DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA to obtain the information of template amount based on fluorescence signals while amplifying whole-genome DNA. The detection limit of real-time MDA was as low as 0.5 pg μl<sup>-1</sup> (5 pg DNA input), offering the whole-genome research a promising tool to quantify the entire DNA during amplification without sacrificing sample completeness or introducing redundant steps."],"journal":["RSC advances"],"pagination":["4617-4621"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8694541"],"repository":["biostudies-literature"],"pubmed_title":["Quantifying genome DNA during whole-genome amplification <i>via</i> quantitative real-time multiple displacement amplification."],"pmcid":["PMC8694541"],"pubmed_authors":["Qiao Y","Lu Z","Luo Y","Long N","Tu J"],"additional_accession":[]},"is_claimable":false,"name":"Quantifying genome DNA during whole-genome amplification <i>via</i> quantitative real-time multiple displacement amplification.","description":"DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA to obtain the information of template amount based on fluorescence signals while amplifying whole-genome DNA. The detection limit of real-time MDA was as low as 0.5 pg μl<sup>-1</sup> (5 pg DNA input), offering the whole-genome research a promising tool to quantify the entire DNA during amplification without sacrificing sample completeness or introducing redundant steps.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-04-22T22:03:00.886Z","creation":"2025-04-06T03:56:18.444Z"},"accession":"S-EPMC8694541","cross_references":{"pubmed":["35424416"],"doi":["10.1039/d0ra09021b"]}}