biostudies-literatureUnknown11(8)Tu JDNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA to obtain the information of template amount based on fluorescence signals while amplifying whole-genome DNA. The detection limit of real-time MDA was as low as 0.5 pg μl-1 (5 pg DNA input), offering the whole-genome research a promising tool to quantify the entire DNA during amplification without sacrificing sample completeness or introducing redundant steps.RSC advances4617-4621https://www.ebi.ac.uk/biostudies/studies/S-EPMC8694541biostudies-literatureQuantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification.PMC8694541Qiao YLu ZLuo YLong NTu JfalseQuantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification.DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA to obtain the information of template amount based on fluorescence signals while amplifying whole-genome DNA. The detection limit of real-time MDA was as low as 0.5 pg μl-1 (5 pg DNA input), offering the whole-genome research a promising tool to quantify the entire DNA during amplification without sacrificing sample completeness or introducing redundant steps.2021-01-01T00:00:00Z2021 Jan2025-04-22T22:03:00.886Z2025-04-06T03:56:18.444ZS-EPMC86945413542441610.1039/d0ra09021b