biostudies-literatureSong NNational Natural Science Foundation of China12885-12892https://www.ebi.ac.uk/biostudies/studies/S-EPMC8697359biostudies-literatureUnknown11(21)Besides geography and climate, biological factors play an important role in shaping travertine landforms, but the biochemical mechanisms of microbial processes in travertine formation have been rarely studied. Two psychrophilic bacterial strains, A20-18 and B21-3 of <i>Pseudomonas psychrophila</i>, isolated from travertine pools of Huanglong, a typical alpine travertine landform, were investigated for their roles in calcium carbonate mineralization, including the deposition process and products. X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy were used to characterize the crystal phase and morphology of CaCO₃ precipitation. The results showed that there were no significant differences between the two strains in CaCO₃ deposiRSC advancesCarbonate biomineralization differentially induced by two psychrophilic <i>Pseudomonas psychrophila</i> strains isolated from an alpine travertine landform.PMC8697359415720354147230941877288Song NYang LHuang YLi QSun GZhou YPan LDong PZhao YZhao XfalseCarbonate biomineralization differentially induced by two psychrophilic <i>Pseudomonas psychrophila</i> strains isolated from an alpine travertine landform.Besides geography and climate, biological factors play an important role in shaping travertine landforms, but the biochemical mechanisms of microbial processes in travertine formation have been rarely studied. Two psychrophilic bacterial strains, A20-18 and B21-3 of <i>Pseudomonas psychrophila</i>, isolated from travertine pools of Huanglong, a typical alpine travertine landform, were investigated for their roles in calcium carbonate mineralization, including the deposition process and products. X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy were used to characterize the crystal phase and morphology of CaCO₃ precipitation. The results showed that there were no significant differences between the two strains in CaCO₃ deposi2021-01-01T00:00:00Z2021 Mar2026-05-31T06:59:56.283Z2025-02-19T01:55:16.447ZS-EPMC86973593542381510.1039/d1ra00578b