biostudies-literatureZhou JJLaboratory for Lingnan Modern AgricultureWuhan Applied Foundational Frontier ProjectChina Agriculture Research System of MOF and MARAThe National Key Research and Development Program1311https://www.ebi.ac.uk/biostudies/studies/S-EPMC8698383biostudies-literatureUnknown10(12)Intermuscular bones (IBs) are small spicule-like bones located in the myosepta of basal teleosts, which negatively affect the edibleness and economic value of fish. Blunt snout bream (<i>Megalobrama amblycephala</i>, with epineural and epipleural IBs) and tilapia (<i>Oreochromis niloticus</i>, without epineural and epipleural IBs) are two major aquaculture species and ideal models for studying the formation mechanisms of fish IBs. Here, we compared myosepta development between <i>M. amblycephala</i> and <i>O. niloticus</i>, based on histological analysis, transcriptome profiling, and expression analysis of bone-related genes. The histological results showed that dye condensation began to appear in the myosepta 20 days post hatching (dph) in <i>M. amblycephala</i>, and IBs could be clearly BiologyComparison of Myosepta Development and Transcriptome Profiling between Blunt Snout Bream with and Tilapia without Intermuscular Bones.PMC869838320200206010122532018YFD0900102CARS-46-08NZ2021011Zhou JJChang YJWang XDGao ZXLiao QChen YLShi RHfalseComparison of Myosepta Development and Transcriptome Profiling between Blunt Snout Bream with and Tilapia without Intermuscular Bones.Intermuscular bones (IBs) are small spicule-like bones located in the myosepta of basal teleosts, which negatively affect the edibleness and economic value of fish. Blunt snout bream (<i>Megalobrama amblycephala</i>, with epineural and epipleural IBs) and tilapia (<i>Oreochromis niloticus</i>, without epineural and epipleural IBs) are two major aquaculture species and ideal models for studying the formation mechanisms of fish IBs. Here, we compared myosepta development between <i>M. amblycephala</i> and <i>O. niloticus</i>, based on histological analysis, transcriptome profiling, and expression analysis of bone-related genes. The histological results showed that dye condensation began to appear in the myosepta 20 days post hatching (dph) in <i>M. amblycephala</i>, and IBs could be clearly 2021-01-01T00:00:00Z2021 Dec2026-04-08T07:06:44.479Z2022-02-11T14:36:52.825ZS-EPMC86983833494322610.3390/biology10121311