biostudies-literaturePang JNIH CTSANCATS NIH HHSNIDCR NIH HHSNIDCD NIH HHSNCI NIH HHSNINDS NIH HHSNIH NIDCRNIGMS NIH HHSNIH NIDCD6772-6786https://www.ebi.ac.uk/biostudies/studies/S-EPMC8710294biostudies-literatureUnknown27(24)<h4>Purpose</h4>Human papillomavirus (HPV) plays a major role in oncogenesis and circular extrachromosomal DNA (ecDNA) is found in many cancers. However, the relationship between HPV and circular ecDNA in human cancer is not understood.<h4>Experimental design</h4>Forty-four primary tumor tissue samples were obtained from a cohort of patients with HPV-positive oropharynx squamous cell carcinoma (OPSCC). Twenty-eight additional HPV oropharyngeal cancer (HPVOPC) tumors from The Cancer Genome Atlas (TCGA) project were analyzed as a separate validation cohort. Genomic, transcriptomic, proteomic, computational, and functional analyses of HPVOPC were applied to these datasets.<h4>Results</h4>Our analysis revealed circular, oncogenic DNA in nearly all HPVOPC, with circular human and human-viral hyClinical cancer research : an official journal of the American Association for Cancer ResearchExtrachromosomal DNA in HPV-Mediated Oropharyngeal Cancer Drives Diverse Oncogene Transcription.PMC8710294R01 NS073831UL1TR001442R01 CA204264T32 GM008806T32 DC000028UL1 TR001442R01 DE023347CA204264-01R01DE023347-04U24 CA264379R01 GM114362Ball LBafna VFisch KSadat SNguyen NGuo TXu GCalifano JFinegersh AFlagg MMischel PSLuebeck JPang JPanuganti BNakagawa TCahill GRen SfalseExtrachromosomal DNA in HPV-Mediated Oropharyngeal Cancer Drives Diverse Oncogene Transcription.<h4>Purpose</h4>Human papillomavirus (HPV) plays a major role in oncogenesis and circular extrachromosomal DNA (ecDNA) is found in many cancers. However, the relationship between HPV and circular ecDNA in human cancer is not understood.<h4>Experimental design</h4>Forty-four primary tumor tissue samples were obtained from a cohort of patients with HPV-positive oropharynx squamous cell carcinoma (OPSCC). Twenty-eight additional HPV oropharyngeal cancer (HPVOPC) tumors from The Cancer Genome Atlas (TCGA) project were analyzed as a separate validation cohort. Genomic, transcriptomic, proteomic, computational, and functional analyses of HPVOPC were applied to these datasets.<h4>Results</h4>Our analysis revealed circular, oncogenic DNA in nearly all HPVOPC, with circular human and human-viral hy2021-01-01T00:00:00Z2021 Dec2026-05-09T03:19:39.733Z2025-02-19T02:57:03.521ZS-EPMC87102943454831710.1158/1078-0432.CCR-21-248410.1158/1078-0432.ccr-21-2484