{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han IC"],"funding":["NEI NIH HHS"],"pagination":["575-579"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8713574"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(10)"],"pubmed_abstract":["<b><i>Purpose:</i></b> Despite numerous recent advances in retinal gene therapy using adeno-associated viruses (AAVs) as delivery vectors, there remains a crucial need to identify viral vectors with the ability to transduce specific retinal cell types and that have a larger carrying capacity than AAV. In this study, we evaluate the retinal tropism of 2 chimeric helper-dependent adenoviruses (HDAds), helper-dependent adenovirus serotype 5 (HDAd5)/3 and HDAd5/35, both <i>ex vivo</i> using human retinal explants and <i>in vivo</i> using rats. <b><i>Methods:</i></b> We transduced cultured human retinal explants with HDAd5/3 and HDAd5/35 carrying an eGFP vector and evaluated tropism and transduction efficiency using immunohistochemistry. To assess <i>in vivo</i> transduction efficiency, subreti"],"journal":["Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics"],"pubmed_title":["Chimeric Helper-Dependent Adenoviruses Transduce Retinal Ganglion Cells and Muller Cells in Human Retinal Explants."],"pmcid":["PMC8713574"],"funding_grant_id":["P30 EY025580"],"pubmed_authors":["Wiley LA","Han IC","Mullins RF","Kaalberg EE","Burnight ER","Boyce TM","Stone EM","Tucker BA","Fingert JH"],"additional_accession":[]},"is_claimable":false,"name":"Chimeric Helper-Dependent Adenoviruses Transduce Retinal Ganglion Cells and Muller Cells in Human Retinal Explants.","description":"<b><i>Purpose:</i></b> Despite numerous recent advances in retinal gene therapy using adeno-associated viruses (AAVs) as delivery vectors, there remains a crucial need to identify viral vectors with the ability to transduce specific retinal cell types and that have a larger carrying capacity than AAV. In this study, we evaluate the retinal tropism of 2 chimeric helper-dependent adenoviruses (HDAds), helper-dependent adenovirus serotype 5 (HDAd5)/3 and HDAd5/35, both <i>ex vivo</i> using human retinal explants and <i>in vivo</i> using rats. <b><i>Methods:</i></b> We transduced cultured human retinal explants with HDAd5/3 and HDAd5/35 carrying an eGFP vector and evaluated tropism and transduction efficiency using immunohistochemistry. To assess <i>in vivo</i> transduction efficiency, subreti","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-05T10:17:18.031Z","creation":"2025-04-05T10:17:18.031Z"},"accession":"S-EPMC8713574","cross_references":{"pubmed":["34597181"],"doi":["10.1089/jop.2021.0057"]}}