biostudies-literatureUnknown102(4)Wang HOne SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and assessment of the impact on diagnostic test performance.Diagnostic microbiology and infectious disease115631https://www.ebi.ac.uk/biostudies/studies/S-EPMC8715644biostudies-literatureA deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2.PMC8715644Lucyshyn JMMagrini VJean SLeber ALWang HWilson RKWilson SAMcGrath SfalseA deletion in the N gene of SARS-CoV-2 may reduce test sensitivity for detection of SARS-CoV-2.One SARS-CoV-2-positive sample demonstrated impaired detection of the N1 target by RT-PCR using US CDC primer/probe sets. A 3 nucleotide deletion was discovered that overlaps the forward primer binding site. This finding underscores the importance of continued SARS-CoV-2 mutation surveillance and assessment of the impact on diagnostic test performance.2022-01-01T00:00:00Z2022 Apr2025-04-26T19:49:17.239Z2022-02-11T16:17:53.624ZS-EPMC87156443504538210.1016/j.diagmicrobio.2021.115631